Inyigisho y'Ubumenyi bw'Impeshyi: Iyo umuraba w'ubushyuhe bwa 40°C uhuye n'igerageza rya molekile

Ubushyuhe bwinshi bwakomeje kugaragara mu gice kinini cy'Ubushinwa vuba aha. Ku ya 24 Nyakanga, Ikigo gishinzwe igenzura ry'ikirere cya Shandong Provincial Meteorological Observatory cyasohoye itangazo ry'ubushyuhe bwinshi bw'umuhondo, riteganya ko ubushyuhe bumeze nk'ubwa sauna buzaba kuri 35-37°C (111-133°F) na 80% by'ubushyuhe mu minsi ine iri imbere mu turere two mu gihugu imbere. Ubushyuhe mu bice nka Turpan, Xinjiang, buri hafi kugera kuri 48°C (111-133°F). Wuhan na Xiaogan, muri Hubei, biri munsi y'ubushyuhe bw'umuhondo, ubushyuhe burenga 37°C mu bice bimwe na bimwe. Muri ubu bushyuhe bukabije, isi ntoya iri munsi y'ubuso bwa pipettes irimo guhura n'ibibazo bidasanzwe - guhagarara neza kwa aside nucleic, imikorere ya enzymes, n'imiterere y'umubiri w'ibinyabutabire byose bihinduka buhoro buhoro kubera ubushyuhe.

Gukura aside nucleique byabaye irushanwa rijyanye n'igihe. Iyo ubushyuhe bwo hanze burenze 40°C, ndetse n'aho icyuma gikonjesha gicanye, ubushyuhe bw'ameza yo gukora akenshi bugera hejuru ya 28°C. Muri iki gihe, ingero za RNA zisigaye mu mwanya ufunguye ziragabanuka inshuro zirenze ebyiri kurusha mu mpeshyi no mu gihe cy'impeshyi. Mu gukuramo aside magnetic bead, umuti w'ubuhumekero uba wuzuye mu gace runaka bitewe no guhinduka vuba kw'umusemburo, kandi kristu zoroshye kugwa. Izi kristu zizatuma habaho guhinduka gukomeye mu mikorere yo gufata aside nucleique. Guhindagurika kw'imisemburo organic byiyongera icyarimwe. Kuri 30°C, ingano ya chloroform volatilization yiyongeraho 40% ugereranije na 25°C. Mu gihe cyo gukora, ni ngombwa kwemeza ko umuvuduko w'umuyaga mu gipfundikizo cy'umuriro ari 0.5m/s, kandi ugakoresha uturindantoki twa nitrile kugira ngo ukomeze kugira ubushobozi bwo kurinda.

Igerageza rya PCR rihura n’ibibazo bikomeye kurushaho by’ubushyuhe. Ibyuma nka enzyme ya Taq na transcriptase ya reverse birakomeye cyane iyo ubushyuhe buhindagurika. Kuzura kw’inkuta z’umuyoboro nyuma yo gukuramo muri firigo ya -20°C bishobora gutuma enzymes zitakaza agaciro ka 15% iyo zinjiye muri sisitemu yo gusubiza. Ibisubizo bya dNTP bishobora kandi kugaragaza ko byangiritse nyuma y’iminota 5 gusa yo guhura n’ubushyuhe bw’icyumba (>30°C). Ikoreshwa ry’igikoresho naryo ribangamirwa n’ubushyuhe bwinshi. Iyo ubushyuhe bwo muri laboratwari buri >35°C kandi ubushobozi bwo gusohora ubushyuhe bw’igikoresho cya PCR budahagije (<50 cm uvuye ku rukuta), itandukaniro ry’ubushyuhe bw’imbere rishobora kugera kuri 0.8°C. Uku kunyuranamo gushobora gutuma ubushobozi bwo kongera imbaraga ku nkengero z’isahani y’amabati 96 bugabanukaho hejuru ya 40%. Imashini ziyungurura ivumbi zigomba gusukurwa buri gihe (kwiyongera kw’ivumbi bigabanya ubushobozi bwo gusohora ubushyuhe ho 50%), kandi icyuma gikonjesha kigomba kwirindwa. Byongeye kandi, iyo ukora igerageza rya PCR mu ijoro ryose, irinde gukoresha igikoresho cya PCR nk' "firigo y’agateganyo" yo kubika ingero. Kubika kuri 4°C mu gihe kirenga amasaha 2 bishobora gutuma amazi ahinduka nyuma y’uko umupfundikizo ushyushye ufunze, bigagabanya uburyo bwo gukora reaction ndetse bigashobora kwangiza modules z’icyuma z’igikoresho.

Mu gihe habayeho imiburo ihoraho ku bushyuhe bwinshi, laboratwari za molekile nazo zigomba gutangaza. Ingero za RNA z'agaciro zigomba kubikwa inyuma ya firigo ya -80°C, aho kwinjira mu bihe by'ubushyuhe bwinshi bigengwa. Gufungura umuryango wa firigo ya -20°C inshuro zirenga eshanu ku munsi bizongera ihindagurika ry'ubushyuhe. Ibikoresho bitanga ubushyuhe bwinshi bisaba nibura cm 50 z'umwanya wo gukwirakwiza ubushyuhe ku mpande zombi no ku mpande z'inyuma. Byongeye kandi, birasabwa kuvugurura igihe cy'igerageza: 7:00-10:00 za mu gitondo ku bikorwa birwanya ubushyuhe nko gukuramo RNA no gushyiramo qPCR; 1:00-4:00 za mu gitondo ku mirimo itari iy'igerageza nko gusesengura amakuru. Iyi ngamba ishobora gukumira neza ko ubushyuhe bwinshi bushobora kubangamira intambwe z'ingenzi.

Igerageza rya molekile mu gihe cy'ubushyuhe ni ikizamini cy'ubuhanga n'ubwihangane. Mu gihe cy'izuba ryinshi ry'impeshyi, wenda igihe kirageze cyo gushyira hasi pipette yawe no kongeramo agasanduku k'urubura mu bipimo byawe kugira ngo igikoresho gishobore gusohora ubushyuhe bwinshi. Uku kubaha ihindagurika ry'ubushyuhe ni cyo kintu cy'agaciro cyane mu gihe cy'izuba ryinshi—n'ubundi kandi, mu bushyuhe bwa dogere selisiyusi 40 mu mpeshyi, ndetse na molekile zikenera "akarere k'impande gakozwe mu buryo bw'ubukorano" karinzwe neza.