Igitabo cyubumenyi bwimpeshyi: Iyo ubushyuhe bwa 40 ° C buhuye nubushakashatsi bwa molekile

Ubushyuhe bwo hejuru bwakomeje kugaragara mu Bushinwa vuba aha. Ku ya 24 Nyakanga, Ikigo cy’ubumenyi bw’ikirere cya Shandong cyatanze integuza y’ubushyuhe bwo hejuru bw’umuhondo, iteganya ko ubushyuhe bwa “sauna” bwa 35-37 ° C (111-133 ° F) n’ubushyuhe bwa 80% mu minsi ine iri imbere mu gihugu cy’imbere. Ubushyuhe ahantu nka Turpan, Sinayi, buregera 48 ° C (111-133 ° F). Wuhan na Xiaogan, Hubei, bari munsi ya orange, ubushyuhe burenga 37 ° C mu turere tumwe na tumwe. Muri ubu bushyuhe bukabije, isi ya microscopique munsi yubuso bwa pipeti ihura n’imivurungano idasanzwe - ituze rya acide nucleique, imikorere ya enzymes, hamwe nuburyo umubiri wa reagent byose bigorekwa bucece nubushyuhe.

Gukuramo aside Nucleic byahindutse isiganwa ryigihe. Iyo ubushyuhe bwo hanze burenze 40 ° C, kabone niyo haba hari icyuma gikonjesha, ubushyuhe bwameza bukora akenshi buzamuka hejuru ya 28 ° C. Muri iki gihe, ingero za RNA zisigaye kumugaragaro zirenze inshuro ebyiri nko mu mpeshyi no mu gihe cyizuba. Mugukuramo amasaro ya magneti, igisubizo cya buffer cyuzuyemo hafi kubera guhindagurika kwihuta kwumuti, kandi kristu ziragwa byoroshye. Iyi kristu izatera ihindagurika rinini muburyo bwo gufata aside nucleic. Ihindagurika ryimyunyu ngugu yiyongera icyarimwe. Kuri 30 ° C, ingano ya chloroform ihindagurika yiyongera 40% ugereranije na 25 ° C. Mugihe cyo gukora, birakenewe kwemeza ko umuvuduko wumuyaga muri fume hood ari 0.5m / s, kandi ugakoresha uturindantoki twa nitrile kugirango ukomeze gukora neza.

Ubushakashatsi bwa PCR buhura nubushyuhe bukabije. Reagent nka Taq enzyme na reverse transcriptase yunvikana cyane nihindagurika ryubushyuhe butunguranye. Kwiyongera ku rukuta rw'igituba nyuma yo gukurwa kuri firigo -20 ° C birashobora gutera igihombo kirenga 15% bya enzyme iyo yinjiye muri sisitemu yo kubyitwaramo. Ibisubizo bya DNTP birashobora kandi kwerekana kwangirika kugaragara nyuma yiminota 5 gusa yubushyuhe bwicyumba (> 30 ° C). Imikorere y'ibikoresho nayo ibangamiwe n'ubushyuhe bwo hejuru. Iyo ubushyuhe bwibidukikije bwa laboratoire ari> 35 ° C kandi gukuraho ubushyuhe bwibikoresho bya PCR ntibihagije (

Guhura nubushyuhe bukabije bwo hejuru, laboratoire ya molekile nayo igomba kuvuza induru. Ingero zagaciro za RNA zigomba kubikwa inyuma ya firigo -80 ° C, hamwe no kugarukira mugihe cy'ubushyuhe bwo hejuru. Gufungura umuryango wa firigo -20 ° C inshuro zirenze eshanu kumunsi bizongera ihindagurika ryubushyuhe. Ibikoresho bitanga ubushyuhe bwinshi bisaba byibura cm 50 z'umwanya wo gukwirakwiza ubushyuhe kumpande zombi no kuruhande. Byongeye kandi, birasabwa kuvugurura igihe cyubushakashatsi: 7: 00-10: 00 AM kubikorwa byubushyuhe bukabije nko gukuramo RNA no gupakira qPCR; 1: 00-4: 00 PM kubikorwa bitari ubushakashatsi nko gusesengura amakuru. Izi ngamba zirashobora gukumira neza ubushyuhe bwo hejuru butabangamira intambwe zikomeye.

Ubushakashatsi bwa molekulari mugihe cy'ubushyuhe ni ikizamini cyubuhanga no kwihangana. Munsi yizuba ridahwema, birashoboka ko igihe kirageze cyo gushyira hasi pipeti yawe hanyuma ukongeramo agasanduku kiyongereyeho urubura kuburugero rwawe kugirango igikoresho kigabanye ubushyuhe bwinshi. Uku kubaha ihindagurika ry'ubushyuhe ni bwo bwiza bwa laboratoire ifite agaciro mu mezi y'izuba ryinshi - erega, mu bushyuhe bwa 40 ° C bwo mu cyi, ndetse na molekile ikenera ubwitonzi “akarere ka polarike.”