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Uturemangingo tw’inyamaswa dushobora kugabanywamo uturemangingo tw’inyuma y’umubiri (epithelial), uturemangingo tw’inyuma (connective), uturemangingo tw’imitsi n’utw’ubwonko bitewe n’inkomoko yatwo, imiterere yatwo, imiterere yatwo n’imikorere yatwo, ibyo bikaba bifitanye isano kandi bifitanye isano mu buryo butandukanye kugira ngo bikore ingingo zitandukanye n’imikorere y’inyamaswa kugira ngo birangize ibikorwa bitandukanye by’umubiri.
Uturemangingo tw’imbere: tugizwe n’uturemangingo twinshi tw’imbere tw’imbere dufatanye neza hamwe n’uturemangingo duto two hagati y’uturemangingo tw’imbere tw’umubiri tumeze nk’ururabo, akenshi tuba turi mu buso bw’umubiri w’inyamaswa no mu mubiri w’imiyoboro itandukanye, imyobo, uduce duto n’imbere mu ngingo zimwe na zimwe. Uturemangingo tw’imbere tw’imbere dufite inshingano zo kurinda, gusohora, gusohora no kwinjiza.
Uturemangingo duhuza: Tugizwe n'uturemangingo n'ingano nini y'uturemangingo. Uturemangingo duhuza twakozwe na mesoderm ni ubwoko bw'uturemangingo tw'inyamaswa dukwirakwira cyane kandi dutandukanye, harimo uturemangingo duhuza, uturemangingo twinshi, uturemangingo duhuza amaso, uturemangingo, uturemangingo tw'amagufwa, uturemangingo tw'amafuta n'ibindi. Dufite inshingano zo gushyigikira, guhuza, kurinda, kwirwanaho, gusana no gutwara.
Imitsi: igizwe n'uturemangingo tw'imitsi dufite ubushobozi bwo kwihagarika. Imiterere y'uturemangingo tw'imitsi ni ntoya nk'umunyu, bityo yitwa kandi umunyu. Akamaro nyamukuru k'umunyu ni ukwihagarika no gukora imitsi. Dukurikije imiterere n'imiterere y'uturemangingo tw'imitsi n'imikorere itandukanye, imitsi ishobora kugabanywamo imitsi y'amagufwa (imitsi inyuranamo), imitsi yoroshye n'imitsi y'umutima.
Uturemangingo tw’imitsi: uturemangingo tugizwe n’uturemangingo tw’imitsi n’uturemangingo tw’imyakura. Uturemangingo tw’imitsi ni uturemangingo tw’imiterere n’imikorere y’urusobe rw’imitsi kandi dufite ubushobozi bwo kumva ibintu bitera imbere n’inyuma no gukora ibyifuzo mu mubiri.
Igicuruzwa cy'ifi nini
Iyi porogaramu ikoresha uburyo bwihariye bwo gufata ADN bwakozwe kandi bunoze kandi buhuza neza, bushobora gufata no gukurura aside nucleic vuba. Ikwiriye gukurura no gutunganya ADN ya genomic mu bwoko bwose bw'inyama n'ingingo z'imbere mu mubiri (harimo n'ibinyabuzima byo mu mazi), kandi ishobora gukuraho ubwoko bwose bwa poroteyine, ibinure n'ibindi bintu by'umwimerere n'indi myanda. Ishobora gukoreshwa hamwe naIfi niniUburyo bwa Magnetic Bead Nucleic Acid Extractor, ikwiriye cyane mu gukuramo ingero nini mu buryo bwikora. Ibikomoka kuri aside nucleic acid bivanywemo bifite ubuziranenge n'ubwiza, kandi bishobora gukoreshwa cyane mu bushakashatsi bwa PCR/qPCR, NGS, Southern hybridisation n'ubundi bushakashatsi bw'igerageza.
Ibiranga:
Ingero nyinshi: ADN ya genome ishobora gukurwa mu bwoko bwose bw'ingero z'inyama z'inyamaswa
Ni nziza kandi ntigira uburozi: reagent ntabwo irimo ibintu by'uburozi nka phenol, chloroform, nibindi, bifite umutekano mwinshi
Kwikora: guhuza na Bigfish Nucleic Acid Extractor bishobora gukoreshwa mu gukuramo umusaruro mwinshi, cyane cyane bikwiranye no gukuramo ingero nini
Ubuziranenge bwinshi: bushobora gukoreshwa mu bushakashatsi bwa biology ya molekile, nka PCR, igogorwa rya enzymes no guhuza ibintu mu buryo butaziguye
Ibikoresho Bikoreshwa: BFEX-32/BFEX-32E/BFEX-96E
Uburyo bwo gukuramo:
Ingero: 25-30mg y'inyama z'inyamaswa
Gusya: gusya azote y'amazi, gusya cyangwa gukata
Igogora: 56℃ igogora rishyushye ryo mu bwogero
Gushyiramo umwuka mu kirere: gukoresha centrifugation kugira ngo ukureho supernatant, no kongeramo ku isahani iri mu mwobo kugira ngo ikuremo amazi mu kirere.
Amakuru y’igerageza: Hafashwe 30mg y’ingero z’uturemangingo twavuye mu bice bitandukanye by’imbeba, hakorwa isuzuma rya ADN hamwe n’amabwiriza ya BFMP01R. Ibisubizo by’igerageza byagaragaje ko agakoresho ka BFMP01R gafite igipimo cyiza cyo gukuramo.
Igihe cyo kohereza: Nyakanga-17-2025
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