Agakoresho ko Gupima Aside ya Nyukleyiki ya SARS-CoV-2 (Fluorescence RT-PCR)

Ibisobanuro bigufi:

Hari primers nshya zihariye kuri virusi ya corona hamwe n'ibikoresho byihariye bipima urumuri muri sisitemu y'ibizamini by'imashini. Acide nshya ya corona-virusi yongerwamo uburyo bwa PCR fluorescent probe in vitro amplification, kandi ibimenyetso bya fluorescent birekurwa mu gihe cy'ibizamini bigenzurwa kandi bigakusanywa hakoreshejwe igikoresho cya fluorescence quantitative PCR, kugira ngo hamenyekane vuba ibisubizo.

TWANDIKIRE

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ibiranga

1. Ububasha bwo kumenya ibintu bwinshi: Imipaka yo kubimenya (LoD) <2 × 102 kopi / ml

2. Ingirabuzimafatizo eshatu: gene ya orflab, gene ya N n'ingirabuzimafatizo y'imbere byabonetse icyarimwe, bikurikiza amabwiriza ya OMS

3. Ikwiriye ibikoresho bitandukanye: ABI7500/7500FAST; Roche LightCycler480; BioRad CFX96; BigFish-BFQP16/48 yacu bwite

4. Byihuse kandi byoroshye: Reagent ivanze mbere yo kuyikoresha biroroshye kuyikoresha, abakiriya bakeneye gusa kongeramo enzyme na template. Igikoresho cyo gukuramo aside nucleique cya Bigfish gihuye neza n'iki gipimo. Ukoresheje imashini ikuramo aside yikora, ni bwo buryo bworoshye bwo gutunganya ingero nini.

5. Uburinzi bw'ibinyabuzima: Bigfish itanga urugero rw'amazi yo kubungabunga virusi kugira ngo yihutishe vuba kugira ngo ababikora bagire umutekano.

Imiterere y'ubwiyongere bw'ingufu za SARS-CoV-2

Agakoresho ko Gupima Aside Nucleique

Icyemezo cya CE-IVD

Izina ry'igicuruzwa	Injangwe. Oya.	Gupakira	Inyandiko
Agakoresho ko Gupima Aside ya Nyukleyiki ya SARS-CoV-2 (Fluorescence RT-PCR)	BFRT06M-24	24T	Uburyo bworoshye bwo kugaragaza ubwiza, bukwiriye ingero zitari nziza cyane
	BFRT06M-48	48T