Agakoresho ko Gupima Aside ya Nyukleyiki ya SARS-CoV-2 (Fluorescence RT-PCR)

Ibisobanuro bigufi:

Agakoresho ko gupima aside nucleicic ka SARS-COV-2 (Fluorescence RT-PCR) gashobora gukoreshwa mu gupima aside nucleicic ya coronavirus nshya, gakoreshwa mu gupima no gusuzuma indwara z’ubwandu bushya bwa coronavirus, kakakoreshwa muri CDC, mu bitaro, muri laboratwari y’abandi, mu kigo cy’ibizamini n’ahandi muri laboratwari z’ivuriro.

TWANDIKIRE

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ibiranga Ibicuruzwa

1, Ububasha bwo kumenya ibintu bwinshi: Umupaka wo kubimenya (LoD)＜kopi 2 × 102 / ml.

2, Gene y’intego ebyiri: Gusuzuma gene ya ORFlab na gene ya N icyarimwe, kubahiriza amabwiriza ya OMS.

3, Ikwiriye ibikoresho bitandukanye: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; BigFish-BFQP96/48 yacu bwite.

4, Byihuse kandi byoroshye: Reagent ivanze mbere yo gukoreshwa biroroshye, abakiriya bakeneye gusa kongeramo enzyme na template. Kit ya Bigfish yo gukuramo aside nucleic ihuye neza n'iki gipimo. Ukoresheje imashini yose ikuramo, yihuta gutunganya ingero nyinshi..

5, Ubuziranenge bw'ibinyabuzima: Bigfish itanga urugero rw'amazi yo kubungabunga virusi kugira ngo yihutishe vuba kugira ngo ababikora bagire umutekano..