Sisitemu ya acicle

Ibisobanuro bigufi:

Gukoresha Ikoranabuhanga rya Magnetike rya Magnetike, hitamo ibikoresho bikwiye birashobora guhita dutandukana no kweza acide ndende ya nucleique mu buryo butandukanye (maraso, tissue, akagari, selile). Igikoresho gifite imiterere myiza igishushanyo mbonera, imikorere yuzuye ya ultraviolet no gushyushya, kandi ecran nini yo gukoraho byoroshye gukora. Numufasha mwiza wo gutahura ubuvuzi bwa lalecular na laboratoire yubumenyi bwa siyansi.

Twandikire Imfashanyigisho

Ibisobanuro birambuye

Ibicuruzwa

Ibicuruzwa biranga ibicuruzwa

Sisitemu yo kugenzura inganda ikora imikorere ihamye kumasaha 24
2, umusaruro mwinshi kandi ufite isuku
3, Gutunganya Acide Autocle Autocle Autoction irashobora gukorwa ku ya 32/96 yingero icyarimwe, kubohorera cyane abashakashatsi
4, gushyigikira reagents birashobora gukoreshwa mu ngero zitandukanye nk'igiswa, ibimera, ibimera, ubutaka bwa fecal, kandi bafite ibisobanuro byinshi bya bagiteri / 16t / 96t / 96t
Guhura nabakiriya batandukanye
5, wenyine wateye imbere porogaramu hamwe na ecran ya ecran ituma imikorere yoroshye kandi byihuse
6, imirasire yangiritse irimbura inkoni ya maguneti hamwe ningero, nimashini ifite ibikoresho bya UV

(Ibisubizo bigeragejwe)

Ibizamini bya electrophores Ibisubizo byumubiri

n'ubutaka nyuma yo gukuramo

(Ibisubizo bigeragejwe)

UU dople yakuyeho isesengura rya QPCR

(Harimo bisanzwe)

(Ibisubizo bigeragejwe)

Ng sample yatumye qpcr isesengura ibisubizo

(Harimo bisanzwe)

Oya	ubwoko	imbaraga	igice	A260	A280	260/280	260/230	icyitegererezo
1	RNA	556.505	μg / ml	13.913	6.636	2.097	2.393	spleen
2	RNA	540.713	μg / ml	13.518	6.44	2.099	2.079	spleen
3	RNA	799.469	μg / ml	19.987	9.558	2.091	2.352	impyiko
4	RNA	847.294	μg / ml	21.182	10.133	2.090	2.269	impyiko
5	RNA	1087.187	μg / ml	27.180	12.870	2.112	2.344	umwijima
6	RNA	980.632	μg / ml	24.516	11.626	2.109	2.329	umwijima