Ni ubuhe buryo bwo gutandukanya no gutunganya aside nucleique?

aside nucleique, harimo ADN na RNA, ni biomolecules z'ingenzi zigira uruhare runini mu bijyanye n'imiterere y'uturemangingo, ibinyabuzima bya molekile, na biotechnology. Ubushobozi bwo gutandukanya no gutunganya izi aside nucleic ni ingenzi mu bikorwa bitandukanye, harimo no gusesengura uburyo zikoreshwa mu gushushanya, gutondeka, no gusesengura imyandikire y'uturemangingo. Sisitemu zo gutunganya aside nucleic zikubiyemo uburyo butandukanye bugenewe gukura no gutunganya aside nucleic mu bipimo by'ibinyabuzima. Iyi nkuru irasuzuma uburyo bwo gutandukanya no gutunganya aside nucleic kandi igaragaza akamaro k'izi sisitemu mu bushakashatsi bwa siyansi bugezweho.

Gusobanukirwa isuku y'aside nucleique

Gutunganya aside nucleic bivuze gukuramo ADN cyangwa RNA mu turemangingo cyangwa mu turemangingo, hagakurikiraho gukuraho imyanda nka poroteyine, lipide, n'indi myanda ya selile. Ubuziranenge n'ubuziranenge bwa aside nucleic zakuwemo ni ingenzi mu ikoreshwa ryazo, kuko imyanda ishobora kubuza imikorere ya enzymes no kugira ingaruka ku buryo bufatika ku bisubizo by'igerageza.

Uburyo busanzwe bwo gutandukanya no gutunganya aside nucleic

Gukuramo Phenol-chloroform:Ubu buryo gakondo bukoresha imiti ikomoka ku bimera mu gutandukanya aside nucleic na poroteyine n'ibindi bice by'uturemangingo. Icyitegererezo kivangwa na phenol na chloroform, bigatuma aside nucleic zicikamo ibice mu gice cy'amazi, mu gihe poroteyine ziguma mu gice cy'ibinyabuzima. Nyuma yo gukurura, igice cy'amazi kirimo aside nucleic kirakusanywa kikanyuzwamo ethanol.

Uburyo bushingiye kuri gel ya silica:Uduce twa silica gel dukoreshwa cyane mu bikoresho byo gusukura aside nucleic acid. Ihame ry'ubu buryo ni uko aside nucleic gel ifatana na silica gel ku rugero ruri hejuru rw'umunyu. Nyuma yo gufatana, imyanda iragezwa, hanyuma aside nucleic ikavanwaho hakoreshejwe amazi make cyangwa agakoresho k'umunyu muke. Ubu buryo burakundwa kuko bwihuse, bukora neza, kandi butanga aside nucleic cleanse nyinshi.

Gusukura amasaro ya rukuruzi:Ubu buryo bukoresha amasaro ya rukuruzi atwikiriwe n'ibintu bihuza aside nucleic. Iyo icyitegererezo kivanze n'amasaro ya rukuruzi, aside nucleic zinjira ku buso bw'amasaro. Amasaro ahita atandukanywa n'umuti hakoreshejwe rukuruzi, bityo akavanaho imyanda. Ubu buryo bukoreshwa mu buryo butandukanye kandi bushobora gukoreshwa mu buryo bwikora, bigatuma bukoreshwa mu gutanga umusaruro mwinshi.

Koromatografiya y'inkingi:Ubu buryo bukubiyemo guca icyitegererezo mu nkingi ya chromatographic irimo icyiciro gihoraho gifata neza aside nucleic. Ubwoko butandukanye bw'inkingi burashobora gukoreshwa, harimo n'izishingiye ku gutandukanya ingano cyangwa amahame yo guhanahana iyoni. Aside nucleic zisohoka mu nkingi, bigatuma icyitegererezo gitunganywa.

Uburyo bwa enzyme:Uburyo bwa enzyme, nk'ubukoresha DNase cyangwa RNase, bushobora gukoreshwa mu kwangiza aside nucleic cyangwa imyanda idakenewe. Ubu buryo bugira akamaro cyane cyane mu gutunganya ingero zikomeye, nk'izirimo ADN na RNA.

Mu gusoza

Gutandukanya aside nucleique no kuyisukura ni intambwe z'ingenzi mu bushakashatsi no mu mikoreshereze yayo mu binyabuzima.Sisitemu zo gusukura aside ya nucleiqueHa abashakashatsi uburyo butandukanye bwo kubona aside nucleique nziza zikwiriye gukoreshwa mu ntangiriro z'uruziga. Byaba hakoreshejwe uburyo gakondo bwo gukuramo phenol-chloroform cyangwa uburyo bugezweho nka silica gel cyangwa magnetic bead cleansing, guhitamo uburyo biterwa n'ibyo igerageza risaba n'imiterere y'icyitegererezo. Hamwe n'iterambere ry'ikoranabuhanga, ubu buryo bwo gutunganya bwakomeje gutera imbere, bunoza imikorere, umuvuduko, n'ubwizerwe, amaherezo byongera ubushobozi bw'abashakashatsi mu bijyanye n'ibinyabuzima.