Ni ibihe bibazo bikunze kugaragara ku bakoresha ikoranabuhanga rya Thermocycler?

 

Ibibazo by'Ubushyuhe Bungana

Ikibazo gikomeye cyane cya thermocycler ni ukudahuza kw'ubushyuhe muri icyo gice. Ubushyuhe budahagije butanga umusaruro uhindagurika wo kongera imbaraga, bigatuma ubuziranenge bw'igerageza bugabanuka.Umuvuduko w'amashanyarazi wa FastCyclermoderi zikoresha ibintu bya Peltier bigezweho na algorithmi zigezweho kugira ngo zigumane ubunini bwa ± 0.2°C ku myobo yose. Ariko, ibikoresho byo gushyushya bisaza, ifu ishaje, cyangwa imyanda yakusanyijwe bishobora kwangiza imikorere uko igihe kigenda gihita.

Ibimenyetso by'ibibazo by'ubushyuhe: Impinduka za PCR mu myanya runaka y'amazi, imiyoboro idahindagurika yo gushonga, cyangwa umusaruro uhindagurika w'ibicuruzwa ku isahani imwe y'icyitegererezo bigaragaza ibibazo bishobora kubaho ku buryo bumwe bisaba gupima ako kanya.

Ikosa ryo gushyushya umupfundikizo

Imipfundikizo ishyushye irinda ko amazi ahinduka, bigatuma habaho kugabanuka kw'ibivange by'ibisubizo kandi bikagabanya imikorere ya PCR. Kunanirwa gushyushya kw'imipfundikizo biri mu bibazo bikunze kugaragara ku bashyushyamipfundikizi ba thermocycler. Ubushyuhe budahagije bw'umupfundikizo butuma amazi ahinduka, mu gihe gushyushya cyane bishobora kwangiza ingero cyangwa kwangiza ibikoresho bya pulasitiki.

Sisitemu zigezweho za FastCycler Thermal Cycler zifite ubushyuhe bushobora guhindurwa (ubusanzwe 100-110°C) hamwe no kugenzura ubushyuhe neza. Gukomeza kubungabunga buri gihe birimo kugenzura uburyo umuvuduko w'umupfundikizo n'ibikoresho byo gupima ubushyuhe kugira ngo harebwe ko bihuye neza kandi ko ubushyuhe bukwirakwira neza.

Igabanuka ry'igipimo cyo kwiyongera

Umuvuduko wo kwihuta utandukanya thermocyclers nziza n'imashini zisanzwe. Uko igihe kigenda gihita, igipimo cyo gushyushya no gukonjesha gishobora kugabanuka bitewe n'ingufu za Peltier, kwangirika kw'abafana, cyangwa ibibazo byo gukonjesha mu buryo bwo gukonjesha. Uku kwangirika kwongera igihe cyo kwiruka kandi bishobora kugira ingaruka ku bushyuhe.

Ibikoresho bya FastCycler Thermal Cycler byo mu rwego rw'umwuga bikomeza kwihuta cyane (4-5°C/isegonda) binyuze mu buryo bwa Peltier bubiri kandi bigacunga neza ubushyuhe. Mu gihe uguze, genzura neza imiterere y'ubushyuhe n'ubukonje, ntabwo ari ingano ntarengwa yo kwihuta gusa.

Ibibazo bya Porogaramu na Interineti

Imashini zigezweho zikoresha thermocycle zihuza porogaramu zigoye zo gukora porogaramu za protocole, kwandika amakuru, no guhuza umuyoboro wa interineti. Ibibazo bisanzwe bya porogaramu birimo:

Ibibazo bya firmware: Bitera impanuka za porogaramu cyangwa ibipimo by'ubushyuhe bitari byo

Kunanirwa kwa USB/Ethernet: Kurinda kohereza amakuru cyangwa kuyagenzura uri kure

Ibibazo byo gukoraho kuri ecran: Bituma porogaramu za protocol zigorana

Ibibazo byo guhuzaHamwe na sisitemu zo gucunga amakuru muri laboratwari (LIMS)

Inganda zikomeye zitanga amakuru mashya ya firmware buri gihe ndetse n'ubufasha bwa tekiniki bushoboka kugira ngo zikemure ibi bibazo vuba.

Kwangirika no Gucika kw'Imashini

Ibice by'umubiri bigenda birushaho kwangirika buhoro buhoro:

Kwanduza bloc: Ingero zamenetse zituma habaho ubushyuhe budakwiranye busaba gusukurwa neza

Kwangirika kw'aho umupfundikizo uherereye: Gufungura kenshi bigabanya intege z'ibice by'imashini

Kunanirwa kw'abafana: Guteza imbere imikorere myiza yo gukonjesha no kongera igihe cyo gukora ingendo

Uburyo bwo gukurura ibyuma bipima: Gutera ibipimo by'ubushyuhe bitari byo bisaba kongera gupima ubushyuhe

Guhindura imiterere y'ikirere

Imashini zose zipima ubushyuhe zikenera kugenzura ubushyuhe buri gihe. Ibyuma bipima ubushyuhe bihinduka mu buryo busanzwe uko igihe kigenda, bishobora guteza amakosa akomeye mu igerageza. Laboratwari z’abahanga zigomba gukora igenzura ry’ubushyuhe buri gihembwe hakoreshejwe ibyuma bipima ubushyuhe byemewe.

IremeUmuvuduko w'amashanyarazi wa FastCyclermoderi zirimo uburyo bwo kwisuzuma bumenyesha abakoresha ibyo bakeneye mu gupima mbere yuko ibibazo bigira ingaruka ku musaruro. Sisitemu zimwe zigezweho zitanga uburyo bwo gupima bwikora bugabanya uburyo bwo gukoresha intoki.

Ingamba zo kwirinda impanuka

Kugabanya ibibazo bya thermocycler binyuze mu kubungabunga byihutirwa:

• Sukura ibikoresho bishyushya buri kwezi ukoresheje imiti isukura ikwiye
• Genzura neza ubushyuhe buri gihembwe ukoresheje ibikoresho by'ikoranabuhanga bipima ubushyuhe
• Vugurura firmware buri gihe kugira ngo ubone uburyo bwo kunoza no gukosora amakosa
• Simbuza ibikoresho bikoreshwa (gaskets z'umupfundikizo, pads z'ubushyuhe) hakurikijwe gahunda y'uruganda
• Komeza guhumeka neza hafi y'ibikoresho kugira ngo ukonje neza

Guhitamo Ibikoresho Byizerwa

Mu gihe uguze thermocyclers, shyira imbere abakora thermocyclers batanga ibi bikurikira:

Garanti zuzuye: Ikubiyemo ibice byombi n'imirimo

Ubufasha bwa tekiniki butanga ibisubizo: Hamwe n'uburyo bwihuse bwo gusimbuza ibice

Amateka yemejwe: Yagaragaje ko yizewe muri laboratwari z'urungano

Kubungabunga byoroshye gukoreshaIbice byoroshye kubona n'inyandiko zisobanutse neza za serivisi

Umwanzuro

Nubwo thermocyclers zishobora kugaragaza imbogamizi zitandukanye mu mikorere, gusobanukirwa ibibazo bisanzwe bifasha mu guhitamo ibikoresho neza no gutegura neza isuku. Gushora imari muri sisitemu nziza za FastCycler Thermal Cycler zifite ibikorwa remezo bikomeye bigabanya igihe cyo gukora kandi bigatanga ibisubizo bihamye kandi byizewe bya PCR. Gusuzuma ikiguzi cyose cyo gutunga—harimo n'ibisabwa mu kubungabunga n'ubwiza bw'inkunga—aho gusuzuma igiciro cyo kugura gusa. Thermocycler ikwiye iba umufatanyabikorwa wizewe wa laboratwari utanga imyaka myinshi y'imikorere idafite ibibazo n'ibyavuye mu bushakashatsi bishobora kongera kugaragara.