Gufungura amabanga yubuzima: Akamaro k'ibicuruzwa bya acide nucleic

Mu murima wa MoleCular MoleCute, ikuramo acide ya nucleic (ADN na RNA) nintambwe y'ibanze, ituma inzira itanga ibisobanuro bitabarika kubushakashatsi bwakozwe mubikoresho bya kamere. Ibicuruzwa byo gukuramo acicleic byahinduye iyi nzira, bikanonosora neza, byizewe, kandi biboneka kubashakashatsi na laboratoire ku isi. Muri iyi blog, tuzasesengura ibi bikoresho, akamaro k'ibice byabo, n'ingaruka zabo ku iterambere rya siyansi.

Acide ya acicction acide?

Acid acideNibikoresho byagenewe kwigunga ADN cyangwa RNA bitaturutse ku byitegererezo bitandukanye, nkamaraso, tissue, ingirabuzimafatizo, ndetse n'icyitegererezo cyibidukikije. Ibi bice bikunze bikubiyemo reagents zose na protocole bakeneye kugirango byorohereze inzira yo gukuramo, kubungabunga abashakashatsi barashobora kubona acide nziza nucleic nucleic nziza cyane.

Gukuramo

Inzira yo gukuramo mubisanzwe ikubiyemo intambwe nyinshi zingenzi: LISS LISIS, kwezwa, no kwishyuza.

Akagari LISSIS: Intambwe yambere nukufungura selile kugirango urekure acide nucleic. Ibi mubisanzwe bigerwaho ukoresheje lysise irimo ibishoboka byose hamwe na enzymes igabanya inzara na poroteyine.

Gusukura: Nyuma yuko acide ya nucleic arekurwa, intambwe ikurikira ni ugukuraho abamuteye nka proteyine, lipid, nundi myanda ya selile. Ibikoresho byinshi bikoresha inkingi za silica cyangwa amasaro ya magneti kugirango uhitemo acide yahing accleic, bityo koza umwanda.

Kurimbura: Hanyuma, acide nucleic yasukuye yazimye muri buffer ibereye, yiteguye gusaba downstream nka PCR, ikurikiranye, cyangwa cloni.

Kuki ukoresha ibikoresho bya aside iricleic?

Gukora: Uburyo bwa acide gakondo nucleic ni ugutwara igihe nakazi. Gukuramo acicclec yo gukuramo bits byoroshya inzira kandi birashobora kuzuza ibikuramo mugihe cyisaha.

Guhuza: Porotokone isanzwe yatanzwe nizi bits imenyesha kororoka no kwizerwa byibisubizo. Ibi nibyingenzi mubushakashatsi aho ukuri kwukuri, nka diagnostique cyangwa ubushakashatsi.

Guhinduranya: Ibikoresho byinshi byateguwe kugirango ukemure ubwoko butandukanye bwicyitegererezo, bigatuma bakwiranye na porogaramu zitandukanye. Waba ukorana nicyitegererezo cyabantu, ibimera bitera, cyangwa imiterere ya mikorobe, birashoboka ko bihutira guhuriza ibyo ukeneye.

Umukoresha Urugwiro: Acide nyinshi za Acicction Ibikoresho birambuye kandi byateguwe byoroshye gukoresha, ndetse no kubashobora kuba bafite uburambe bwa laboratoire. Ibi byatumye deman cyangwa democratied the tekiniki ya MoleCumillege, yemerera abashakashatsi benshi kwitabira ubushakashatsi bwa genetike.

Gushyira mu bikorwa acide ya acide

ACID ya nucleic yabonetse muriyi bice irashobora kuba ishingiro rya porogaramu nyinshi:

Ubushakashatsi bwa gene: Sobanukirwa imikorere ya gene, imvugo no kugenga.
Gupima ubuvuzi: gutahura indwara zindwara za genetike, indwara zanduza na kanseri.
Siyanse yubucamanza: Isesengura rya ADN zibyitegererezo bya ADN byo gukora iperereza kubyaha.
Ibinyabuzima byubuhinzi: Iterambere ryibinyabuzima byahinduwe na genetike (GMOS) kugirango byongere umusaruro wibihingwa.
Mu gusoza
Acid acideNibikoresho byimpapuro zibinyabuzima bigezweho, bigatuma abashakashatsi bafungura amabanga yubuzima kurwego rwa molekile. Imikorere yabo, guhuzagurika, no guhinduranya kwahinduye imiterere yubushakashatsi bwa genetike no gupima, bigatuma byoroshye kuruta mbere hose kugirango usuzume ADN na RNA. Mugihe ikoranabuhanga rikomeje gutera imbere, dushobora kwitega ko ibyo bikoresho byo guhinduka kurushaho, gufungura imiryango mishya kubitabo bya siyansi no guhanga udushya. Waba umushakashatsi w'inararibonye cyangwa gushya mu murima, gushora mu kikoresho cyiza cya acide gishobora kuzamura imibereho yawe no gutanga umusanzu ku bumenyi bw'iteka ryose muri Genetics.