Kurekura imbaraga z'urubuga rw'amabuye: Igikoresho cy'ingenzi kuri biltechnology

Mu murima wa biologiya na molecular hamwe na biotechnology, imburagihe cyubushyuhe ni ibikoresho byingenzi. Bakunze kwita imashini ya PCR, ibi bikoresho bigira uruhare runini muri ADLA, bituma bikomeza imfuruka yubushakashatsi bwa genetike, gupima ubushakashatsi bwa genetike, gupima, hamwe nibisabwa bitandukanye mubuvuzi nubuhinzi. Gusobanukirwa imikorere n'akamaro k'abanyamperi mu bushyuhe birashobora kumurika ingaruka ziterambere rya siyansi.

Ubushyuhe bw'amabuye y'agaciro ni iki?

A Ubushyuhe Byclernigikoresho cya laboratoire gikora inzira ya polymerese (PCR). PCR ni tekinike ikoreshwa mu rwego rwo kongera ibice byihariye bya ADN, bituma abashakashatsi batanga miliyoni za kopi zikurikiranye. Iyi sanzure ningirakamaro kubintu bitandukanye, harimo cloning, gusesengura gene isesengura, no gukurura genetike.
Ubushyuhe bwubushyuhe bukoresha binyuze murukurikirane rwimpinduka zubushyuhe, zikaba zinabikomeye mubyiciro bitandukanye bya PCR. Izi mpande zirimo Gucana, Gukamba, no Kuramya. Mugihe cyo gusenyuka, ADN yashizweho kabiri irashyuha, igatandukanya imirongo ibiri imwe. Ubushyuhe noneho bwomanuwe mugihe cyicyiciro cya Anneling kugirango yemere Primers guhambira intego ya ADN. Hanyuma, ubushyuhe bugenda bwongeye kwinjiza icyiciro cyo kwihara, aho ADN Polymeyese ya syntheses imirongo mishya ya ADN.

Ibiranga nyamukuru bya cycler

Ubushyuhe bugezweho bugezweho bufite ibintu bitandukanye byongera imikorere yabo no kudashobora gukoreshwa. Kimwe mu iterambere ryingenzi ni ubushobozi bwo gutegura ubushyuhe bwinshi, butuma abashakashatsi bahitamo protocole zabo za PCR. Ubushyuhe bwinshi burimo ubushyuhe kandi burimo inyamanswa zihamye zibuza congensation mugukora ibibyitseho, kugirango ibintu byiza bishoboke kugirango bikoro.
Ikindi kintu kigaragara nukwishyira hamwe kwigihe gito cya PCR imikorere. Inzora zubushyuhe nyayo zifasha abashakashatsi gukurikirana inzira yongerewe mugihe nyacyo, zitanga amakuru yumubare kumera ya ADN. Iyi mikorere ni ingirakamaro cyane muburyo bwa PCR (QPCR), aho ibipimo nyabyo binini binegura kubona ibisubizo nyabyo.

Gusaba Thermal Cycler

Ibisabwa byo ku nshuro zubushyuhe biragutse kandi biratandukanye. Mu kwisuzumisha amavuriro, bakoreshwa mu kumenya indwara zamashanyarazi, ihinduka rya genetike, n'indwara zarazwe. Kurugero, mugihe cya Covidic-Pandemic cycle, ubushyuhe bwagize uruhare runini mugupima byihuse, gufasha kumenya abantu banduye no kugenzura ikwirakwizwa rya virusi.
Muri laboratoire yubushakashatsi, ukwezi kwubushyuhe nibyingenzi kuri gene, bikurikirana, no kuvugisha imvugo. Bemerera abahanga bashakisha itandukaniro rya genetike no kumva uburyo bwindwara. Byongeye kandi, muri ibinyabuzima bishingiye ku binyabuzima, impfizi zubushyuhe zikoreshwa mugutezimbere ibinyabuzima byahinduwe mu buryo bwa kamere (GMOS) bishobora kwihanganira imihangayiko y'ibidukikije cyangwa byongereye ibintu by'intungamubiri.

Ejo hazaza hyrmal cycle

Mugihe ikoranabuhanga rikomeje guhinduka, niko impyisi. Guhanga udushya nka miniaturisation no kwishyira hamwe na platforms ya digitale biri kuri horizon. Izi nyungu ziteganijwe gukora ubushyuhe bugera ku bushyuhe kandi bufite urugwiro, bemerera abashakashatsi, bemerera abashakashatsi gukora ubushakashatsi bukabije kandi butari ukuri.
Byongeye kandi, kuzamuka kw'ibinyabuzima bya Synthetike n'umuti bwite birashobora guteza imbere iterambere ry'ikoranabuhanga rya cycler cycler. Mugihe abashakashatsi bashaka gukoresha neza ibikoresho bya geneti, hakenewe imbuga zamabuye zubushyuhe zikoreshwa muburyo bwo guhuza protocole igoye biziyongera gusa.

Mu gusoza

TheUbushyuhe Bycler birenze igikoresho cya laboratoire; ni irembo ryo gusobanukirwa ubuzima bwuzuye kurwego rwa molekile. Ubushobozi bwayo bwo kongera ADN yahinduye imirima iva mumiti mubuhinzi, ikabigira igikoresho cyingenzi mu gukurikirana ubumenyi no guhanga udushya. Kureba ejo hazaza, nta gushidikanya ko nta gushidikanya ko bizakomeza kugira uruhare runini mu guhindura umurima wa biotechnology na molecular.