Ubwinshi nakamaro kibyapa byimbitse muri laboratoire igezweho

Mwisi yisi igenda itera imbere yubushakashatsi nubushakashatsi, ibikoresho nibikoresho bikoreshwa muri laboratoire bigira uruhare runini mugutsinda kwimishinga itandukanye. Kimwe muri ibyo bikoresho byingirakamaro ni isahani yimbitse. Aya masahani yihariye yabaye ngombwa-muri laboratoire nyinshi, cyane cyane mubice nka biologiya ya biologiya, ibinyabuzima, no kuvumbura ibiyobyabwenge. Muri iyi blog, tuzareba uburyo butandukanye nakamaro k’amasahani yimbitse, imikoreshereze yabyo, nibyiza bazanira abashakashatsi.

Isahani yimbitse ni iki?

A isahani yimbitseni microplate hamwe nuruhererekane rwamariba, buriwese yagenewe gufata ingano nini yamazi kuruta microplate isanzwe. Isahani yimbitse isanzwe ikozwe muri plastiki yujuje ubuziranenge kandi iza muburyo butandukanye hamwe nubushobozi bwiza buri hagati ya ml 1 na ml 50 cyangwa zirenga. Aya masahani yashizweho kugirango yemererwe kubika neza icyitegererezo, kuvanga, no gusesengura, kubigira igikoresho cyingenzi muri laboratoire nyinshi.

Gukoresha isahani yimbitse

Isahani yimbitse ifite intera nini ya porogaramu, harimo ariko ntabwo igarukira kuri:

1. Ububiko bw'icyitegererezo: Abashakashatsi bakunze gukoresha amasahani yimbitse kugirango babike igihe kirekire ingero z’ibinyabuzima nka ADN, RNA, poroteyine, n’umuco w’akagari. Ninini yubushobozi bwiza, icyitegererezo gishobora kubikwa nta ngaruka zo guhumeka cyangwa kwanduzwa.
2. Kugenzura cyane: Mu kuvumbura ibiyobyabwenge no kwiteza imbere, amasahani yimbitse ni ngombwa kugirango harebwe ibicuruzwa byinshi (HTS). Bafasha abashakashatsi gupima ibihumbi icyarimwe icyarimwe, byihutisha cyane kumenya abakandida ibiyobyabwenge.
3. PCR na qPCR: Isahani yimbitse isanzwe ikoreshwa muburyo bwa polymerase yerekana (PCR) hamwe na PCR (qPCR). Byashizweho kugirango bishoboke gusiganwa ku magare neza kandi bigabanye ingaruka zo kwanduzanya hagati yintangarugero.
4. Intungamubiri za poroteyine: Muri biologiya yubatswe, amasahani yimbitse akoreshwa mubushakashatsi bwa protein. Ibyobo binini bitanga umwanya uhagije wo gukura kwa kristu, ni ngombwa mu bushakashatsi bwa X-ray.
5. Umuco w'akagari: Isahani yimbitse nayo ikoreshwa mumasoko yumuco mubidukikije bigenzurwa. Igishushanyo cyabo cyemerera imirongo myinshi yingirabuzimafatizo guhuzwa icyarimwe, byorohereza ubushakashatsi nubushakashatsi.

Ibyiza byo gukoresha amasahani yimbitse

Gukoresha amasahani yimbitse atanga ibyiza byinshi byongera imikorere nukuri muri laboratoire yawe:

1. Kongera ubushobozi: Inyungu nyamukuru yibisahani byimbitse nubushobozi bwabo bwo gufata ingano nini yamazi, ifasha cyane cyane mubushakashatsi busaba urugero rwinshi.
2. Mugabanye ibyago byo kwanduza: Igishushanyo cyibisahani byimbitse bigabanya ibyago byo kwanduzanya hagati yintangarugero kandi byemeza ubusugire bwibisubizo byubushakashatsi.
3. Guhuza na Automation: Amasahani menshi yimbitse arahuzwa na sisitemu yo gutunganya ibintu byikora, bigafasha gukora cyane kandi bigabanya ubushobozi bwikosa ryabantu.
4. Porogaramu zitandukanye: Nkuko byavuzwe mbere, amasahani yimbitse arashobora gukoreshwa mubikorwa bitandukanye, bigatuma igikoresho kinini kubashakashatsi mubyiciro byinshi.
5. Ikiguzi: Mugutunganya ibyitegererezo byinshi icyarimwe, amasahani yimbitse arashobora kubika umwanya numutungo, amaherezo akazigama ibikorwa bya laboratoire.

mu gusoza

Mu gusoza,amasahani yimbitseni igice cyingenzi mubikorwa bya laboratoire bigezweho. Guhindura kwinshi, kongera ubushobozi, no guhuza na automatike bituma baba igikoresho ntagereranywa kubashakashatsi mubice bitandukanye. Mugihe ubushakashatsi bwa siyansi bukomeje gutera imbere, akamaro k’amasahani maremare azagenda yiyongera gusa, bizatanga inzira yubuvumbuzi bushya. Waba ufite uruhare mu kuvumbura ibiyobyabwenge, ibinyabuzima bya molekuline, cyangwa ubundi bumenyi bwa siyansi, gushora imari mu byapa byujuje ubuziranenge birashobora kuzamura ubushobozi bwawe bwubushakashatsi.