Guhindura hamwe n'akamaro k'isahani nziza-maremare muri laboratoire igezweho

Mu isi ihindagurika ku isi yubushakashatsi nubushakashatsi bwa siyansi, ibikoresho nibikoresho bikoreshwa muri laboratoire bigira uruhare runini mugutsinda kwimishinga itandukanye. Imwe mubisobanuro byingenzi ni isahani yimbitse. Izi masahani yihariye yabaye iyambere-ifite muri laboratoire nyinshi, cyane cyane mumirima nka solekile ya molecular, ibinyabuzima, hamwe nibiyobyabwenge. Muri iyi blog, tuzasesengura ibisobanuro n'akamaro k'isahani y'ibyapa cyane, gusaba, nibyiza bazana kubashakashatsi.

Nisahani yimbitse?

A isahani yimbitseni microplate ifite urukurikirane rwinyamanswa, buri kimwe cyagenewe gufata amajwi manini yamazi kuruta microplate isanzwe. Amasahani maremare asanzwe akozwe muri plastiki nziza kandi uze muburyo butandukanye hamwe nubushobozi bwiza buva kuri ml 1 kugeza kuri 50 cyangwa birenga. Izi masahani zagenewe kwemerera ububiko bwicyitegererezo, kuvanga, no gusesengura, kubigira igikoresho cyingenzi muburyo bwinshi bwa laboratoire.

Gusaba isahani yimbitse

Amasahani yimbitse afite uburyo butandukanye bwo gusaba, harimo ariko ntibigarukira kuri:

1. Kubika icyitegererezo: Abashakashatsi bakunze gukoresha amasahani menshi yububiko bwigihe kirekire cyo kubika ingero zibinyabuzima nka ADN, RNA, Proteyine, na Proteines, na selile. Ubushobozi bunini bwuzuye, icyitegererezo cyicyitegererezo kirashobora kubikwa nta kamaro ko guhumeka cyangwa kwanduza.
2. Kugenzura Byinshi: Mubisobanuro byibiyobyabwenge niterambere, amasahani yimbitse nibyingenzi muburyo bwo kwerekana hejuru (hts). Batuma abashakashatsi bagerageza ibihumbi icyarimwe icyarimwe, bihutisha cyane kumenyekanisha abakanyita ibiyobyabwenge.
3. PCR na QPCR: Amasahani yimbitse akoreshwa mubice bya Polymeyese (PCR) na pcr (QPCR) Porogaramu. Bagenewe gushoboza amagare meza yo gusiganwa ku magare kandi bagabanya ibyago byo kwanduza hagati yicyitegererezo.
4. Proteine ​​Crystallsation: Mubinyabuzima byubatswe, amasahani yimbitse akoreshwa kuri poroteine ​​igerageza. Ibyobo binini bitanga umwanya mwiza kuri kristu yo gukura, ni ngombwa kuri X-Ray Crystallography ubushakashatsi.
5. Umuco w'akagari: Isahani yimbitse nayo ikoreshwa mumuco mubidukikije. Igishushanyo cyabo cyemerera imirongo myinshi ya selile igomba gucogora icyarimwe, koroshya kwiga no mubushakashatsi.

Ibyiza byo gukoresha amasahani menshi

Ukoresheje amasahani yimbitse atanga ibyiza byinshi byongera imikorere kandi mubyukuri muri laboratoire yawe:

1. Kongera ubushobozi: Ibyiza byingenzi byisahani nziza nubushobozi bwabo bwo gufata amajwi manini, bifitiye akamaro cyane mubushakashatsi busaba icyitegererezo kinini.
2. Mugabanye ibyago byo kwanduza: Igishushanyo cyisahani yimbitse kigabanya ibyago byo kwanduza hagati yo kwanduza ingero no kureba ubusugire bwibisubizo byubushakashatsi.
3. Guhuza no kwikora: Amasahani menshi yimbitse ahuye na sisitemu yo gutunganya amazi yikora, igaha akazi-kwiyangiza cyane no kugabanya ubushobozi bwikosa ryabantu.
4. Porogaramu: Nkuko byavuzwe haruguru, amasahani yimbitse arashobora gukoreshwa muburyo butandukanye, kubakora igikoresho kidasanzwe kubashakashatsi hejuru yindeyinshi.
5. Igiciro cyiza: Mugutunganya ingero nyinshi icyarimwe, amasahani yimbitse arashobora kubika umwanya nubutunzi, amaherezo uzigame ibikoresho byo gukora laboratoire.

Mu gusoza

Mu gusoza,amasahani menshini igice cyingenzi mubikorwa bya laboratoire bigezweho. Guhinduranya kwabo, kongera ubushobozi, no guhuza no kwikora no kwikora bituma bibamo ibikoresho bitagereranywa kubashakashatsi muburyo butandukanye. Nubushakashatsi bwa siyansi bukomeje gutera imbere, akamaro k'isahani yimbitse izakura neza, ituma inzira yo kuvumbura hashya no guhanga udushya. Waba ufite uruhare mu kuvumbura ibiyobyabwenge, ibinyabuzima bya molecular, cyangwa indi disipulini iyo ari yo yose, ishoramari mu masahani meza cyane yo kuzamura cyane ubushobozi bwawe bwubushakashatsi.