Igitabo Cyingenzi Kuri 8-Strip PCR Ibiyobora: Guhindura Laboratwari Yakazi

Mubyerekeranye na biologiya ya biologiya, neza kandi neza nibyingenzi byingenzi. Igikoresho kimwe gitezimbere cyane imikorere ya laboratoire ni 8-plex PCR. Imiyoboro mishya yashizweho kugirango yoroshe inzira ya polymerase ikora (PCR), ituma abashakashatsi bakora ubushakashatsi byoroshye kandi neza. Muri iyi blog, tuzasesengura ibyiza bya 8-plex ya PCR, sisitemu yo gusaba, hamwe nuburyo bwo kongera ubushobozi bwabo muri laboratoire.

Imiyoboro 8 ya PCR ni iki?

8-gukuramo imirongo ya PCRbikozwe mubitereko umunani bitandukanye bya PCR bihujwe murukurikirane kugirango bibe umurongo. Igishushanyo cyemerera ingero nyinshi kwongerwa icyarimwe, bigatuma bikwiranye cyane nubushakashatsi bwimbitse. Buri muyoboro wa PCR muri strip tube urashobora gufata ingano yihariye yimvange ya reaction, mubisanzwe miriyoni 0.1 kugeza kuri 0.2 ml, ikwiranye nibikorwa bitandukanye bya PCR.

Inyungu zo gukoresha imirongo 8 ya PCR

1. Kunoza imikorere: Imwe mu nyungu zingenzi zo gukoresha 8-strip ya PCR ni ukubika icyitegererezo cyo gutegura. Aho gukoresha imiyoboro ya PCR kugiti cye, abashakashatsi barashobora gupakira icyarimwe icyarimwe icyarimwe, bikagabanya ibyago byo kwanduzwa namakosa yabantu.
2. Ubukungu kandi neza: Ukoresheje ibizamini, laboratoire irashobora kugabanya umubare wibikenerwa bikenewe mubushakashatsi. Ibi ntibigabanya ibiciro gusa, ahubwo binagabanya ingaruka za plastiki zikoreshwa kubidukikije.
3. Kunoza icyitegererezo cyo gukurikirana: Imiyoboro myinshi ya 8-PCR izana ahantu hagaragara neza, bituma abashakashatsi bamenya byoroshye ingero. Iyi mikorere ningirakamaro mubigeragezo aho icyitegererezo gikwiye ari ingenzi kugirango habeho kubyara.
4. Automatisation irahuye: Mugihe laboratoire igenda ikoresha tekinoroji yo gukoresha, igishushanyo mbonera cya 8-imirongo ya PCR nayo irahuza na sisitemu yo gukoresha. Uku guhuza byongera ibicuruzwa kandi bigashyigikira ibishushanyo mbonera bigoye.
5. Guhindagurika: 8-imirongo ya PCR irashobora gukoreshwa mubikorwa bitandukanye, harimo PCR yuzuye (qPCR), kwandukura inyandiko PCR (RT-PCR), na genotyping. Ubwinshi bwayo butuma iba igikoresho cyingenzi muri laboratoire nyinshi yibinyabuzima.

Gukoresha 8-imirongo ya PCR

Porogaramu ya 8-strip PCR ya tebes iragutse kandi iratandukanye. Bikunze gukoreshwa kuri:

• Kwipimisha kwa Clinical: Muri laboratoire zubuvuzi, imiyoboro 8 ya PCR irashobora gukoreshwa kugirango tumenye vuba indwara zandura, indwara zikomoka ku moko, hamwe na kanseri.
• Ubushakashatsi n'iterambere: Mubushakashatsi bwubushakashatsi ninganda, utu tubari dufite agaciro kubushakashatsi bwerekeranye na genetiki, guteza imbere urukingo, nibindi bikorwa bya biologiya.
• Ubumenyi bwubucamanza.

Inama zo gukoresha cyane ikoreshwa rya 8-strip PCR

1. Hindura uburyo bwo kwitwara: Menya neza ko imiterere ya PCR itezimbere kubushakashatsi bwawe bwihariye. Ibi birimo guhindura ubushyuhe bwa annealing, igihe cyo kwaguka, hamwe na enzyme yibanze.
2. Koresha reagent nziza: Intsinzi ya PCR iterwa ahanini nubwiza bwa reagent yakoreshejwe. Gusa muguhitamo ADN polymerase yo murwego rwohejuru, primers, na buffers birashobora kuboneka ibisubizo byizewe.
3. Komeza kutabyara: Kugira ngo wirinde kwanduza, burigihe ukoreshe tekinike ya aseptic mugihe ukoresha imiyoboro 8 ya PCR. Ibi birimo kwambara uturindantoki, gukorera ahantu hasukuye, no kwirinda kwanduza umusaraba hagati yintangarugero.
4. Kubika neza: Bika imiyoboro idakoreshwa 8-PCR ahantu hakonje, humye kugirango ukomeze ubusugire bwabo. Kurikiza amabwiriza yo kubika ibicuruzwa.

mu gusoza

8-gukuramo imirongo ya PCRni tekinoroji ihungabanya mubijyanye na biologiya ya molekuline, hamwe nibyiza byinshi bishobora kuzamura imikorere ya laboratoire kandi neza. Mugusobanukirwa ibyiza byayo nibisabwa, abashakashatsi barashobora gukoresha ibyo bikoresho kugirango banoze akazi kandi babone ibisubizo byizewe. Waba ukora isuzuma rya clinique, ubushakashatsi bwa siyanse cyangwa isesengura ryubucamanza, kwinjiza imiyoboro 8 ya PCR mumikorere ya laboratoire yawe irashobora kunoza imikorere yawe neza. Emera ejo hazaza ha PCR hanyuma urebe ubushakashatsi bwawe butera imbere hamwe niki gisubizo gishya!