Impinduramatwara Ubushakashatsi: sisitemu nyayo ya PCR

Mw'isi ya biologiya na genetiki, sisitemu-nyayo PCR yagaragaye nkumukino uhindura umukino, uhindura uburyo abashakashatsi basesengura no kubahiriza acide nucleic. Iyi mikino yo guca ahagaragara yatumye inzira zingenzi ziterambere mu mirima nko gusuzuma ubuvuzi, gukurikirana ibidukikije, no guteza imbere ibiyobyabwenge. Muri iyi nyandiko ya Blog, tuzasenya ibintu byingenzi bya sisitemu yigihe cya PCR, dushakisha ubushobozi bwayo, porogaramu, n'ingaruka byagize kubushakashatsi bwa siyansi.

Gusobanukirwa Ikoranabuhanga ryikoranabuhanga rya PCR

Igihe nyacyo PCR, uzwi kandi nka PCR CCR (QPCR), ni tekiniki zikomeye za molecular zikoreshwa mu kongera no icyarimwe zigereranya molekile ya ADNED. Bitandukanye na PCR gakondo PCR, itanga igipimo cyujuje ubuziranenge cya ADNA, PCR nyayo PCR yemerera gukurikirana inzira yo kwanze mugihe nyacyo. Ibi bigerwaho binyuze mugukoresha ibinure bya fluorescent cyangwa ibizamini bisohora ikimenyetso nkuko ADNPlifification Yongerewe igenda itera imbere. Thesisitemu-nyayo pcr sisitemuifite ibikoresho hamwe nibikoresho byihariye na software ifasha gupima no gusesengura amakuru yo kwagura, atanga abashakashatsi bafite ibisubizo byumvikana kandi byizewe.

Porogaramu mu gusuzuma kwa muganga

Imwe mubisabwa byingenzi bya sisitemu yigihe cya PCR muri sisitemu yo gusuzuma ubuvuzi. Iri koranabuhanga ryagize uruhare runini mu gutahura no guhuza indwara ya virusi nka virusi, bagiteri, na fungi. Mu rwego rw'indwara zanduza, PCR nyayo PCR ifasha kumenyekanisha byihuse kandi byoroshye kumenyekanisha microbial abakozi ba mikorobe, bakemerera gusuzuma hakiri kare no gutabara ku gihe. Byongeye kandi, PCR-Igihe cya PCR cyabaye gihamye mugukurikirana imvugo ya gene ijyanye nindwara zitandukanye, zitanga ubushishozi bwingenzi muburyo bwa molekisi niterambere.

Gukurikirana ibidukikije n'ubushakashatsi

Sisitemu yigihe cya PCR nayo yasanze ikoreshwa cyane mugukurikirana ibidukikije nubushakashatsi. Kuva mu gusuzuma mikorobe itandukanye mu butaka no ku ngero zo gukurikirana ikwirakwizwa ry'ibinyabuzima byahinduwe mu buhinzi, PCR nyayo PCR itanga igikoresho gisanzwe cyo gusesengura aside iriclex. Byongeye kandi, iri koranabuhanga ryagize ubuco mu kumenya ibidukikije hamwe n'impuguke zigamije kurinda ibidukikije n'ubuzima rusange.

Ingaruka ku iterambere ry'ibiyobyabwenge n'ubushakashatsi

Mu rwego rwo guteza imbere ibiyobyabwenge n'ubushakashatsi, sisitemu nyayo PCR yakinnye uruhare rukomeye mu gusuzuma ibiyobyabwenge, uburozi, na farumasi. Mugushoboza uburyohe bwa gene ya gene na ADN / RNA intego, PCR nyayo yorohereza isuzuma ryimpinduka ziterwa nibiyobyabwenge kurwego rwa molekile. Ibi bifite ingaruka kumiti yihariye, nkuko PCR nyayo PCR ishobora gufasha mukumenya imiterere itandukanye igira ingaruka ku miti yihariye, bityo igayobora ingamba zo kwivuza no kunoza umusaruro wihanga.

Ibizaza hamwe niterambere

Mugihe ikoranabuhanga rikomeje guhinduka, sisitemu-nyayo ya PCR yiteguye gutera imbere, kuzamura ubushobozi bwayo no kwagura ibyifuzo byayo. Imbaraga zubushakashatsi zikomeje kwibanda ku kuzamura imitekerereze, ubushobozi bwo gukusanya, no gufatanya kwibibuga nyabyo PCR, hagamijwe gukora ikoranabuguzi nyayo, hagamijwe gukora ikoranabuhanga kugerwaho kandi bwumukoresha. Byongeye kandi, kwishyira hamwe kwa PCR nyayo hamwe nubundi buryo bwo gusesengura, nko gukurikiranya ibisekuruza bikurikira, bisezeranya gufungura imipaka mishya mubisesengura hamwe na molecular.

Mu gusoza, Uwitekasisitemu-nyayo pcr sisitemuihagaze nk'ibifuni by'ibinyabuzima bya molecular ba none kandi byasize ikimenyetso kidahari ku bushakashatsi bwa siyansi. Ubushobozi bwayo bwo gutanga isesengura ryihuse, ryukuri, kandi ryumubare ryateye imbere mu murima utandukanye, kubera ubuvuzi muri siyanse y'ibidukikije. Mugihe abashakashatsi bakomeje gukora imbaraga zukuri PCR, turashobora gutegereza inzira nyayo izahindura ejo hazaza h'ibinyabuzima bishingiye ku binyabuzima.