Guhindura PCR: Umuvuduko w'amagare yihuta

Mu rwego rwa biologiya ya biologiya,Amagare yubushyuheni ibikoresho by'ingenzi kubashakashatsi n'abahanga. Bafite uruhare runini mubikorwa bya polymerase reaction (PCR), niyo shingiro ryo kwongera ADN, gukoroniza no gusesengura ibintu bitandukanye. Mu basiganwa ku magare menshi ku isoko, FastCycler igaragara neza hamwe n'ibiranga iterambere ryayo ndetse n'ibigize ubuziranenge, bihinduka icyitegererezo cyo guhanga udushya no gukora neza.

Intandaro ya FastCycler niyemeza ubuziranenge, ikoresheje ibintu byiza bya Peltier byo muri Marlow, muri Amerika. Ibi bintu bizwiho kwizerwa no gukora, bituma FastCycler igera ku gipimo gitangaje cy’ubushyuhe bugera kuri 6 ° C / S. Ubu bushobozi bwihuse ningirakamaro mukugabanya igihe cyose gisabwa kugirango amagare ya PCR, atume abashakashatsi babona ibisubizo byihuse bitabangamiye ubusugire bwubushakashatsi.

Kimwe mu bintu byihuta bya FastCycler ni imibare yacyo itangaje, irenga miliyoni 100. Uku kuramba bivuze ko abashakashatsi bashobora gukoresha FastCycler mugihe kirekire, bigatuma ishoramari rihendutse muri laboratoire zisaba gusiganwa ku magare bikomeza kandi bigasubirwamo. Kuramba kwa FastCycler ni gihamya yubuhanga bwayo nubuhanga, byemeza ko ishobora kwihanganira imikoreshereze ya laboratoire ya buri munsi.

Ubushyuhe burakomeye mubikorwa bya PCR, kandi FastCycler irusha abandi muriki kibazo. Hamwe na tekinoroji igezweho yo gushyushya no gukonjesha hamwe na PID (proportional-integral-derivative) igenzura ubushyuhe, FastCycler ikomeza urwego rwo hejuru rwubushyuhe mugihe cyamagare. Uku kuri ni ingenzi kugirango umuntu agere kuri ADN neza, kuko no gutandukana kwubushyuhe bishobora gutera ibisubizo bibi cyangwa gutsindwa kwubushakashatsi.

Guhuza amariba yose nubundi buryo bukomeye bwo gusiganwa ku magare, kandi FastCycler ntabwo itenguha. Igishushanyo cyacyo cyemeza ko ingero zose zishyuha kandi zigakonja buri gihe, zikaba ari ingenzi kubushakashatsi busaba ibintu bimwe. Ubu bumwe bugabanya guhinduka mubisubizo, biha abashakashatsi ikizere ko amakuru yabo yizewe kandi yororoka.

Mubyongeyeho, FastCycler ikora kurwego rwo hasi rwurusaku, bigatuma iba nziza kubidukikije bya laboratoire bisaba umwuka utuje. Iyi mikorere ntabwo itezimbere gusa abashakashatsi kumikorere, ahubwo inatuma uburambe bwa laboratoire bwibanze kandi neza.

Muri make ,.Amagare yihutabyerekana iterambere ryinshi mubuhanga bwa PCR. Nibikoresho byayo byiza cyane bya Peltier, igipimo cyihuta cyihuta, icyerekezo cyiza cyamagare, hamwe nuburyo bugezweho bwo kugenzura ubushyuhe, byateguwe kugirango bihuze ibyifuzo byubushakashatsi bwibinyabuzima bigezweho. Waba ukora ibikorwa bya ADN byiyongera cyangwa ukora ubushakashatsi bugoye, FastCycler itanga imikorere ihanitse, yizewe, kandi ikora neza. Gushora imari muri FastCycler bisobanura gushora imari mugihe kizaza cyubushakashatsi bwawe, kwemeza ko ufite ibikoresho ukeneye kugirango uhindure imipaka yubuvumbuzi bwa siyansi.