Guhindura PCR: Umuvuduko w'amagare yihuta

Mu rwego rwa biologiya ya biologiya,Amagare yubushyuhe nigikoresho cyingirakamaro mubikorwa bya polymerase reaction (PCR). Mugihe abashakashatsi na laboratoire bakurikirana imikorere nukuri, FastCycler yabaye umukino uhindura umukino murwego. Hamwe na tekinoroji igezweho kandi ikora neza, FastCycler ishyiraho ibipimo bishya byo gusiganwa ku magare.

FastCycler ikoreshwa nibikoresho byiza bya Peltier byo muri Marlow, muri Amerika. Ibi bikoresho byateguwe neza kugirango bitange ubushyuhe buhebuje, hamwe nubushyuhe bwo hejuru yubushyuhe bugera kuri 6 ° C / s. Ubu bushobozi bwihuse bugabanya cyane igihe gikenewe kuri buri cyiciro cya PCR, bigatuma abashakashatsi barangiza ubushakashatsi vuba bitabangamiye ubuziranenge.

Kimwe mu bintu byihuta bya FastCycler ni imibare yacyo itangaje, irenga miliyoni 100. Uku kuramba kwemeza ko laboratoire zishobora gukoresha FastCycler igihe kirekire, bigatuma ishoramari rihendutse kubigo byose byubushakashatsi. Ubuzima burebure bwa FastCycler bivuze ko abashakashatsi bashobora kwibanda kubushakashatsi bwabo batiriwe bahangayikishwa no kunanirwa kw'ibikoresho cyangwa gukenera gusimburwa kenshi.

Ubushyuhe burakomeye muri PCR, kandi FastCycler iruta iyindi ngingo. Ukoresheje ubushyuhe bugezweho bwo gushyushya no gukonjesha, hamwe na PID (proportional-integral-derivative) igenzura ubushyuhe, FastCycler ikomeza urwego rwo hejuru rwubushyuhe mugihe cyose cyamagare. Uku kuri ningirakamaro kugirango tugere ku bisubizo byizewe kandi byororoka, ni ngombwa mu bushakashatsi bwa siyansi.

Guhuza amariba yose nubundi buryo bukomeye bwo gusiganwa ku magare, kandi FastCycler ntabwo itenguha. FastCycler yashizweho kugirango irebe neza ubushyuhe buri gihe kuri buri riba, bigabanya ingaruka zo guhinduka mubisubizo bya PCR. Uku guhuza ni ngombwa cyane cyane mugihe ukorana nicyitegererezo gisaba amplification nyayo, kuko ifasha kwemeza ko ibisubizo byose bikorwa mubihe bimwe.

Mubyongeyeho, FastCycler ikora ituje, ikora neza muri laboratoire isaba ahantu hatuje. Iyi mikorere ituma abashakashatsi bakora ubushakashatsi batabangamiwe n urusaku rwimashini, bigatera umwuka wibanze kandi utanga umusaruro.

Usibye ubuhanga bwa tekinike, FastCycler yateguwe hitawe kubakoresha-inshuti. Imigaragarire yimbere nuburyo bworoshye bwo gutangiza porogaramu byorohereza abashakashatsi babimenyereye hamwe nabashya gukoresha. Ubushobozi bwo gukora protocole no gukurikirana byoroshye iterambere byongera ubunararibonye bwabakoresha, bituma abahanga bibanda kubushakashatsi bwabo aho gukoresha imashini igoye.

Muri make, ByihutaUmukinnyi w'amagarebyerekana iterambere ryinshi mubuhanga bwa PCR. Hamwe nubwiza bwayo bwiza bwa Peltier, kwihuta cyane, icyerekezo cyiza cyamagare, hamwe no kugenzura ubushyuhe bwambere, bitanga imikorere ntagereranywa kubikorwa bya biologiya ikoreshwa. Gukomatanya kwukuri, uburinganire, hamwe n urusaku ruke rutuma FastCycler igikoresho cyingenzi muri laboratoire iyo ari yo yose igamije kubona neza ibisubizo byiza. Mugihe icyifuzo cya PCR cyihuse kandi cyizewe gikomeje kwiyongera, FastCycler igaragara nkumuyobozi murwego, bituma abashakashatsi bahindura imipaka yubuvumbuzi.