Impinduramatwara PCR: Kwiyiriza ubusa Ubushyuhe Bycler

Mu murima wa MoleCular MoleCuling,cycle ni igikoresho cyingenzi muri Polymeyese reaction (PCR). Nkuko abashakashatsi na laboratoire bakurikirana neza kandi neza, kwiyiriza ubusa byabaye umukinamikino mumurima. Hamwe na tekinoroji yaka kandi imikorere myiza, yihuta itanga amahame mashya yo gusiganwa ku maguru.

Igiyisweraho gikoreshwa nubunini bwikinyabuhanga buturuka bwa Marlow, muri Amerika. Ibi bintu byateguwe neza gutanga ubushyuhe buhebuje bwubushyuhe, ubushyuhe bwa ramp igipimo cya 6 ° C / S. Iyi mvugo ya Ramp Ramp cyane igabanya igihe gisabwa kuri buri GCR Gyr, kwemerera abashakashatsi kugirango babuze ubushakashatsi bwihuse utabangamiye.

Kimwe mu bintu byihuta byagaragaye nibibara bitangaje byanyuma, birenga miliyoni 100. Iri baramba ryemeza ko Labozi zirashobora gukoresha igisibo igihe kirekire, bigatuma ishoramari ryiza ryikigo icyo aricyo cyose cyubushakashatsi. Uburebure burebure cyane busobanura ko abashakashatsi bashobora kwibanda ku bushakashatsi bwabo batagomba guhangayikishwa no kunanirwa kw'ibikoresho cyangwa gukenera gusimburwa kenshi.

Ubushyuhe bwukuri ni ingenzi muri PCR, kandi igisibo cyitwaye neza muriki kibazo. Gukoresha techmoelect Technoelectric no Gukonjesha ikoranabuhanga, ihujwe na pide (bigereranywa-bikomokaho - bikomokamo) kugenzura ubushyuhe, ibiyiko bikomeza urwego rwo hejuru rwukuri muburyo bwo gusiganwa ku magare. Uku kunegura kunegura kugirango tugere kubisubizo byizewe kandi byororoka, byingenzi mubushakashatsi bwa siyansi.

Ubusambanyi hakurya yose nubundi buryo bukomeye bwo gusiganwa ku magare, kandi igisibo ntirutenguha. Igisibo cyagenewe kwemeza umwirondoro wubupfura uhamye kuri buri soko, kugabanya ibyago byo guhinduka mubisubizo bya PCR. Ubuhungiro ni ngombwa cyane cyane mugihe ukorana nibyitegererezo bisabwa byoroshye, nkuko bifasha kwemeza ko ibitekerezo byose bikorwa mubihe bimwe.

Byongeye kandi, icyiciro cyihuta cyane, bigatuma ari byiza kuri laboratoire zisaba akazi gatuje. Iyi mikorere yemerera abashakashatsi gukora ubushakashatsi badahuje urusaku rwimashini, bakora ikirere cyibanze kandi gitanga umusaruro.

Usibye ibisobanuro bya tekiniki, ikiyiko cyashizweho n'umukoresha-urugwiro mubitekerezo. Interineti yacyo yo kwitera hamwe na gahunda yoroshye yoroshye yorohereza abashakashatsi bafite uburambe naba Novice kugirango bakoreshe. Ubushobozi bwo gutunganya protocole no gukurikirana inzira byoroshye imbere yongera uburambe bwumukoresha, bituma abahanga bibanda kubushakashatsi bwabo aho gukora imashini igoye.

Muri make, kwiyiriza ubusaUbushyuhe Byclerbyerekana iterambere rikomeye muri tekinoroji ya PCR. Hamwe na peltier yo hejuru yuburinganire, gukinisha byihuse, imbaraga nziza zo gusiganwa ku magare, no kugenzura ubushyuhe buteye imbere, itanga imikorere itagereranywa kuri porogaramu ya molecular. Ihuriro ryukuri, ubumwe, hamwe nubusa bwurusaku butuma yihuta igikoresho cyingenzi kuri laboratoire iyo ari yo yose igamije kubona neza ibisubizo byiza. Nkibisabwa PCR yihuta kandi yizewe ikomeje kwiyongera, igisibo kigaragara nkumuyobozi mumurima, ushoboza abashakashatsi kugirango basunike imipaka yubuvumbuzi bwa siyansi.