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Kuva indwara ya Coronavas yo muri 2019 (Covid-19) icyorezo cya Acide ya Acide Ubucuruzi (Naats) yatejwe imbere ku isi kandi yabaye isuzuma risanzwe. Nubwo ibizamini byinshi byatejwe imbere kandi bigakoreshwa mubizamini byo gusuzuma laboratoire, imikorere yibi bigeragezo ntabwo yasuzumwe muburyo butandukanye. Kubwibyo, ubu bushakashatsi bwari bugamije gusuzuma imikorere ya Abbott-Cov-2, Daan Gene, BGI, na Sanse bihuza ibikoresho byo mu bibuga ukoresheje ibipimo ngenderwaho (CRS). Ubushakashatsi bwakorewe mu kigo cy'ubuzima rusange cya Etiyopiya (Efurayi) kuva 1 kugeza ku ya 30 Ukuboza 2020. Cy'ibihe 164, 59.1% byari byiza kandi 40.9% byari bibi kuri CRS. Umuyoboro wa Sansure wa Sansech wari muto cyane ugereranije na crs (p <0.05). Umuyoboro wa Sansure wa Sansech wari muto cyane ugereranije na crs (p <0.05). Положительнытытыты Sansure biotech Бар Biotech Б.чительно по сравнениюю Сmvikana (P <0,05). Ibisubizo byiza bya Sansech byagabanutse cyane ugereranije na CRS (P <0.05).与 CRS 相比, Sansure biotech 的阳性率显着较低 (P <0.05).与 CRS 相比, Sansure biotech 的阳性率显着较低 (P <0.05). У Sansure ibinyabuzima Б. чнаитело меньшерелоних результатов по сравнениюю с crs (P <0,05). Sansure bioki yari afite ibisubizo bike cyane ugereranije na crs (p <0.05).Amasezerano rusange yo gusesengura bine yari 96.3-100% ugereranije na CRS. Usibye umubare muto wa sanse ya Sanse Fitech assuay, imikorere y'ishuri rine yagereranijwe hafi. Nkibyo, Sansech [Ubushakashatsi gusa (Ruo) gusa (Ruo) gusa] isuzuma bisaba izindi cyemezo kugirango zikoreshwe muri Etiyopiya. Hanyuma, ubushakashatsi bwinyongera bugomba gufatwa nko gusuzuma ibishuko hamwe nibirego byabigenewe.
Kwipimisha kwa Laboratoire ni umwe mu bagize umuryango w'ubuzima ku isi (NEHO) Indwara ya Coronavirus 2019 (Covid-19) Gutegura no gusubiza (SPCP). Abagira inama ko ibihugu bigomba kubaka ubushobozi bwa laboratoire bwo kuzamura itegurwa, imiyoborere iboneye, kuba maso no gusubiza vuba ku bibazo by'ubuzima rusange. Ibi byerekana ko uruhare rwa laboratoire ari urufunguzo rwo kuranga indwara nicyorezo cyabakozi banduye banduye no kugenzura ikwirakwizwa ryabo.
Gusuzuma Covise-19 bisaba amakuru yibyopisoji nubuvuzi, ibimenyetso / ibimenyetso, hamwe na radiyo na laboratoire. Kubera ko icyorezo cya Cuhan-19 mu Bushinwa, acide acide mu bucuruzi mu bucuruzi (naats) yatejwe imbere ku isi. Igihe nyacyo gisubirwamo guhinduranya urunigi (RRT-PCR) cyakoreshejwe nkuburyo busanzwe kandi busanzwe bwo gusuzuma laboratoire ya laboratoire ya laboratoire ya laboratoire ya laboratoire ya Gressrome 2 (SARV-COV-2) 3. Gutahura molekati-COV-2 mubisanzwe bishingiye kuri n (gene ya NucleoCapsid Gene), e (Ihatiro ya RNA (RNA-Proteine (RNA-Prolymeye / B (ifunguye gusoma 1a / b). Gene) akarere kagaragaye muri genone ya virusi. Bafatwa nkuturere nyamukuru twabitswe dusanga muri genomen ya virusi kugirango tumenye virusi4. Muri izo genzure ya RDRP na e zifite ibyiyumvo byo gusesengura byimazeyo, mugihe gene ifite sennitivite nkeya isesengura.
Imikorere ya PCR isaba irashobora gutandukana bitewe nibintu bitandukanye nka: Gukuramo reagent, byongera / gutahura / uburyo bwo gukuramo, uburyo bwo gukuramo, ubwiza bwimashini ya PCR nibindi bikoresho. Kugeza ku ya 20 Mata Muri Etiyopiya, hakoreshwa inshuro 14 PCR PCR ikoreshwa mu kumenya SCR-COV - 2 mu bigo nderabuzima 26, birimo Abi 7500, Abbott, Abbott, muri Roche 48000 na Mediyo7. Byongeye kandi, ibizamini bitandukanye bya PCR birahari, nk'ikigeragezo cya Daan Gene, Abbott SARS-COV-2 ikizamini cya Sansure, na Sars-COV-COV-2 Ikizamini cya BGI. Nubwo RRT-PCR yumve cyane, abarwayi bamwe bifitanye isano na Covid-19 raporo y'ibinyoma ibinyoma bitewe na kopi idahagije ya Acide idakwiye ya Acide, Ubwikorezi, Ububiko no Gukemura, no Gukemura imiterere nibikorwa byabakozi8. Mubyongeyeho, icyitegererezo cyangwa kugenzura nabi, kuzenguruka. CT) Rero, biragaragara ko ibizamini bya PCR bishobora rwose kumenya abatwara ibice bya gene, kuko bidashobora no gutandukanya genes zingenzi, bityo ibizamini birashobora kumenya gusa abatwara gusa kandi ntabwo ari abarwayi10. Kubwibyo, ni ngombwa gusuzuma imikorere yo gusuzuma ukoresheje uburyo busanzwe mugushiraho. Nubwo bidahwitse ya Naat iraboneka mu kigo cy'ubuzima rusange cya Etiyopiya (Efurayi) ndetse no mu gihugu hose, nta suzuma rigereranya ry'ikigereranyo cyabo ntiriramenyekana. Kubwibyo, ubu bushakashatsi bwari bugamije gusuzuma imikorere igereranya yubucuruzi buboneka mubucuruzi bwo kumenya SARS-Crt-PCR hakoresheje ingero zivuriro.
Abatabiriye 164 bakekwaho gukekwaho Covid-19 bashyizwe muri ubu bushakashatsi. Abenshi mu ngero zari zo ziva mu bigo bivururwa (118/164 = 72%), mu gihe abitabiriye 46 (28%) bakomoka mu bigo bidavunwa. Mu bitabiriye amahugurwa ntabwo yavuwe mu kigo, 15 (9.1%) yakenyeje imanza na 31 (18.9%) yari afite imanza zemeza. Ibice mirongo cyenda na bitatu (56.7%) abitabiriye) bari abagabo, kandi bivuze (± SD) imyaka y'abitabiriye imyaka 31.10 (± 11,482).
Muri ubu bushakashatsi, ibiciro byiza kandi bibi by'ibizamini bine kuri Covid-19 byari byagenwe. Rero, igipimo cyiza cya Abbott Sars-Cov-2 Assay Gene 2019-NCOV Assure, 58.5%, 57.9% na 55.5%. Ibyiza kandi bibi bigizwe nibisanzwe (CRS) amanota yari 97 (59.1%) na 67 (40.9%), kubijyanye (imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe (Imbonerahamwe 1). Muri ubu bushakashatsi, ibisobanuro bya CRS byari bishingiye ku mategeko "ari yo yose", aho tuba ibisubizo bine by'ikizamini, ibisubizo bibiri cyangwa byinshi byatanze umusaruro umwe byafatwaga nkibyiza cyangwa bibi.
Muri ubu bushakashatsi, twabonye amasezerano mabi (NPA) ya 100% (95% Ci 94.6-100) kubisesengura ugereranije na CRS. The Sansure Biotechnology analysis showed a minimal PPA of 93.8% (95% CI 87.2-97.1) and the Daan Gene 2019-nCoV analysis had an overall agreement of 99.4% (95% CI 96.6-99.9). Ibinyuranye, amasezerano rusange hagati ya sars-cov-2 bgi assuy na sansech 2014-NCOV ifata 98,8% na 96.3%, kubijyanye (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe (imbonerahamwe 2).
Cohen's Kappa Coeefficient ya Cohen hagati ya CRS na Abbott SArs-COV-2 Ibisubizo byangiza byuzuye (k = 1.00). Mu buryo nk'ubwo, KAPAP's KAPAPA indangagaciro zagaragaye na Daan Gene 2019-NCOV, Sars-Cov-2 BGi, na Sanse bihuye neza na CRS (K ≥ 0,925). Muri iri sesengura rigereranya, ikizamini cya Chi-kare (ikizamini cya McNemar) cyerekanaga ko Sanse Fitech 2019-NCOV Ibisubizo byashyizwe ahagaragara na CRS ibisubizo (P = 0.031) (Imbonerahamwe 2).
Nkuko bigaragara ku gishushanyo.1 Ijanisha rya CT Agaciro CT (<20 CT) ya Abbott SArs-COV-2 ifatanije na 87.6% na ORF1% Agaciro ka CT 50.3% (36-40 ct) yari 3,2%. 1 Ijanisha rya CT Agaciro CT (<20 CT) ya Abbott SArs-COV-2 ifatanije na 87.6% na ORF1% Agaciro ka CT 50.3% (36-40 ct) yari 3,2%.Nkuko bigaragara ku gishushanyo.1. анализа Sansure Biotech 2019-NCOV поробазонт кия зантавя 50,3%, А тавя 50,3 3,2%. 1, ijanisha rya CT Agaciro CT (<20 ct) isesengura rya Abbott-CEV-2 (hamwe na 8,3% ya CT Agaciro CT 50.3%, na CT ya 36-40 CT (36-40 ct) yabazwe 3.2%.如图 1 所示, Abbott Sars-Cov-2 检测 (结合 rdrp 和 n 基因) 的最低 ct 值百分比 (<20%, 高 ct 值 (36-40 ct) 的百分比为 3.2%. Nkuko bigaragara ku gishushanyo cya 1, CT yo hasi ya CT yagaciro (<20 CT) ya Abbott SArs-CEV-NCOV Ikizamini cya Sansure ni 50.3%, 高 ct 值 (36-40 ct) 的3.2%. Как покано на دисуне 1, аналид 87,6%, А зачие ct ген1a / b в в вледованид у у поккий CT. Nkuko bigaragara ku gishushanyo cya 1, Abbott SArs-COV-2 ifatanije (guhuza leta ya RDRP na NES) kuri 87,6%, mugihe cya NEF1A / MU GIHE CYA NCOV yerekanye NCOV yerekanye NCOV yerekanye CT yo hasi. Процент значий (<20 ct) Составил 50,3%, А пробилт высокий CT (36-40 ct) Стставил 3,2%. Ijanisha ryindangagaciro (<20 ct) yari 50.3%, hamwe nijanisha rya CT Fight PN (36-40 CT yari 3,2%.Ikizamini cya Abbott-COV-2 B Ikizamini cyanditseho CT Princes Hejuru 30. Ku rundi ruhande, kuri BGI SARS-COV-2 Assury Orf1A / B gene yari ifite agaciro ka CT (> 36 ct) ijanisha rya 4% (Ishusho 1). Ku rundi ruhande, kuri BGI SARS-COV-2 Assury Orf1A / B gene yari ifite agaciro ka CT (> 36 ct) ijanisha rya 4% (Ishusho 1). С Другой стоны, в анализе арен Orf1 Ku rundi ruhande, mu isesengura rya BGI SARS-COV-2 ORF1A / B yari ifite agaciro ka CT (> 36 ct), ijanisha ryayo ryari 4% (Ishusho 1).另一方面, 在 BGI SERS-COV-2 检测中, orf1a / b 基因具有高 ct 值 (> 36 ct) 的百分比为 4% (图 1). Kurundi ruhande, muri BGI SARS-COV-COV-COV-COV - Ijanisha rya Orf1a / B gene ufite agaciro ka CT (> 36 CT) ni 4% (Ishusho 1). С другой стоны, в анализе ау-2 проеними CT (> 36 CT) Составил 4% (Р р. 1). Ku rundi ruhande, muri SARS-2 COV-2 Isesengura, ijanisha rya ORF1A / B BYIZA RIKURIKIRA CT (> 36 CT) yari 4% (Ishusho 1).
Muri ubu bushakashatsi, twafashe ingero 164 nasopharyngeal. Kubwoko bwose bwabanyeshuri, bwo kwigunga bwa RNA na KNLAbishishishishishwa ukoresheje uburyo nibikoresho byasabwe nabakora.
Ubu bushakashatsi bwerekanye ko ikizamini cya Abbott kuri Sars-COV-2 gifite imikorere imwe yo gutahura nka CRS, hamwe na 100%, bibi, kandi muri rusange. Amasezerano ya Kappa's Kappa ni 1.00, yerekana amasezerano yuzuye hamwe na CRR. Ubushakashatsi nk'ubwo bwa Kaminuza ya Washington muri Amerika yasanze kwiyumvisha ibintu bikomeye kandi byihariye ku kizamini cya Abbot kuri SARS-COV-2% na 100%, ugereranije na laboratoire - PLA) ya CDC. 11. Sisitemu ya Abbott-COV-2 ya leta ishingiye kuri icyarimwe yahuye na gen ya n na gen Inyigisho i Vienne, Otirishiya yagaragaje kandi ko gukuramo binini byerekana umubyibumbe wo kumenya no gutahura inshuro nyinshi kugabanya ingaruka zo kwirukana no kongera imenyekanisha ry'ibitekerezo13. Rero, umukino mwiza wa Abbott kuri SARS-COV-2 isuzuma rishobora kubahirizwa na sisitemu yo kumenya icyarimwe ingirabuzimafatizo zabaringaniza, zikuramo umubare munini wibipimo (0.5 ml), no gukoresha umubare munini wa eluent (40 μl).
Ibisubizo byacu kandi byerekanaga ko imikorere yo kumenya ikizamini cya Daan genetique yari hafi nkimwe ya CRS. Ibi bihuye ninyigisho14 yakorewe muri kaminuza ya Anhui muri Huainan, mu Bushinwa, hamwe nuwabikoze amasezerano meza 100%. Nubwo hari amakuru abonye ibisubizo bihamye, icyitegererezo kimwe cyari kibi nyuma yo gusubiramo ibintu bimwe, ariko byari byiza muri SARS-COV-2 na Sansech NCUVE NCUV-2019 isaba. Ibi byerekana ko hashobora kubaho imico itandukanye muburyo butandukanye bwabanyeshuri. Nubwo bimeze bityo ariko, mu bushakashatsi bwakozwe mu Bushinwa15, ibisubizo bya Daan Gene Gen Massay yatandukanijwe cyane (P <0.05) ugereranije na lab-yasobanuwe na laboratoire. Nubwo bimeze bityo ariko, mu bushakashatsi bwakozwe mu Bushinwa15, ibisubizo bya Daan Gene Gen Massay yatandukanijwe cyane (P <0.05) ugereranije na lab-yasobanuwe na laboratoire. Тем не не менее, в мследовани, проведенном в Калитатарьно отличался (P <0.05) ит их Лабораторного эТалононононононого анализа. Ariko, mu bushakashatsi mu Bushinwa15, Isesengura rya Daan Gene ya Dise itandukanijwe cyane (P <0.05) kuva isesengura rya laboratoire yabo.然而, 在中国进行的研究中 15, 大安基因检测的结果与其实验室定义的参考检测相比有显着差异 (p <0.05).然而, 在中国进行的研究中 15, 大安基因检测的结果与其实验室定义的参考检测相比有显着差 <0.05 Однако в иследованитованитом в Китае15, билгияы сравнениюю С лалонон лннаторнымум тетом. Ariko, mu bushakashatsi mu Bushinwa15, ibisubizo by'ikizamini cya Daan Daan byari bitandukanye cyane (p <0.05) ugereranije n'ikizamini cya Laboratoire.Ubu buryo bushobora guterwa no kumva ikizamini cyerekana kugirango tumenye SARS-COV-2, kandi izindi nyigisho zishobora kuba ingenzi kumenya impamvu.
Byongeye kandi, ubushakashatsi bwacu bwasuzumye imikorere igereranya ya Sars-COV-2 BGI isaba ko CRS, yerekana amasezerano meza y'ijanisha (PPA = 97%), na Muri rusange), na Opa). ). = 98.8%). Indangagaciro za KAPAPA zerekanaga amasezerano meza (k = 0.975). Ubushakashatsi mu Buholandi16 n'Ubushinwa15 bwerekanye ibisubizo bihamye. Sars-Cov-2 Ikizamini cya BGI ni gene imwe (orf1a / b) ikizamini cyo kumenya ukoresheje 10 μl Yongerewe / Kumenya Guhuza. Nubwo amasezerano meza yibarurishamibare hamwe nibisubizo byacu, isesengura ryabuze ingero ebyiri nziza (1.22%) yicyitegererezo. Ibi birashobora kugira ingaruka nini zo kuvuza imbaraga zoherezwa haba murwego rwo kubarwayi nabaturage.
Ikindi giterane gishinzwe isesengura kirimo ubu bushakashatsi ni sansure biotech NCOV-2019 Rrt-PCR (Ruo) isaba; Ijanisha rusange ryahuye ni 96.3%. Imbaraga zamasezerano nazo zagenwe no guha agaciro ka Cohen, cyari 0.925, zerekana amasezerano yuzuye hamwe na CRS. Na none kandi, ibisubizo byacu birasa nukwiga byakorewe muri kaminuza yo muri kaminuza nkuru yo hagati i Changsha, no mu ishami rya laboratoire y'amavukire y'ibitaro by'abatavuga, Liuzhou, Umujyi wa Liuzhou, Ubushinwa17. Nubwo handitswe ikibazo cyavuzwe haruguru cyanditswe, ikizamini cya Chi-kare (ikizamini cya macnemar) cyerekanaga ko ibisubizo bya softch bilitech bivuga kuri CRS (P. 0.005). Nubwo handitswe ikibazo cyavuzwe haruguru cyanditswe, ikizamini cya Chi-kare (ikizamini cya macnemar) cyerekanaga ko ibisubizo bya softch bilitech bivuga kuri CRS (P. 0.005). Несмотря на то, что Барикированое ускошее Хогорий یу Хоотерат (Крадрий Макнемара) показал, что Резул Nubwo amasezerano meza y'ibarurishamibare iri hejuru yanditswe, ikizamini cya Chi-kare (ikizamini cya McNemar) cyerekanaga ko ibisubizo bya sotech biotech bigabanyijemo itandukaniro rikomeye ugereranije na CRS (P. 0.005).尽管记录了上述良好的统计一致性, 但卡方检验 (macnemari 检验) 表明, Sansure y'ibiotch 检测的结果与 CRS 相比具有统计学显着差异 (p <0.005).尽管 记录 了 上述 良好 统计 一致性 一致性 一致性 一致性 一致性 ((macnemaring bitionch 表明 显着 显着 ((p <0.005 ...................)))))) Несмотря на Отмеченное хорошее статистистичич Статистически зачимю разницу (P <0,005) между анализом Sansure biotech и CRS. Nubwo amasezerano meza y'ibaruriraho yavuzwe haruguru, ikizamini cya Chi-kare (ikizamini cya McNemar) cyerekanaga itandukaniro rikomeye (P. 0,005) hagati ya Sanse Fitech isaba kandi CRS.Ingero zitandatu (3.66%) zasangaga ari ibinyoma ugereranije na CRS (Imbonerahamwe yinyongera 1); Ibi ni ngombwa cyane, cyane cyane guha imbaraga zo kwanduza virusi. Amakuru yavuzwe haruguru nayo ashyigikira iki gipimo cyo kumenya 2005.
Muri ubu bushakashatsi, CT indangagaciro zagenwe kuri buri gitsina na platifomu, hamwe no hasi cyane bivuze ko agaciro ka CT kavuzwe muri SARS-COV-2. Ibisubizo birashobora kuba bifitanye isano na Abbott icyarimwe bipima geneti yo kugerageza gutahura SARS-COV-2. Kubwibyo, ukurikije Igishushanyo 1, 87,6% bya Abbott Sars-Cov-2 Ibisubizo byari bifite indangagaciro munsi ya 20. Gusa umubare muto wibisubizo byingutsi (12.4%) byari intera 20-30. CT indangagaciro hejuru ya 30 ntabwo byanditswe. Usibye gukoresha imiterere ya SARS-COV-2 Ikibanza gishobora kuba gifitanye isano no kugabanya ibintu byo hasi (32.5 RNA / ML) 18, biruta inshuro eshatu ntarengwa ya kopi 100 ya RNA / ML. ml) 19.
Ubu bushakashatsi bufite aho bugarukira: Ubwa mbere, ntabwo dufite uburyo busanzwe / nka virusi ya virusi cyangwa ibindi bizamini bya laboratoire (LDA)] kubera kubura amikoro. Icya kabiri, ingero zose zikoreshwa muri ubu bushakashatsi harimo swab ya Nasopharyngeal, mu gihe ibisubizo bitakurikijwe ku bundi bwoko bukomeye, n'icya gatatu, Ingano yacu yari nto.
Ubu bushakashatsi bwagereranije imikorere ya ert-PCR isaba kuba Sars-Cov-2 ukoresheje ingero za Nasopharyngeal. Gusaba byose byashishikarizwa byari hafi yimikorere igereranijwe, usibye sansech bimenyewe na Sansure. Byongeye kandi, igipimo cyoroshye cyoroshye cyagaragaye muri Sansure bioch bifatanije na CRS (P <0.05). Byongeye kandi, igipimo cyoroshye cyoroshye cyagaragaye muri Sansure bioch bifatanije na CRS (P <0.05). Кроме того, в Byongeye kandi, ikizamini cya sofubure cya Sanseki cyerekanaga ijanisha rito bwibisubizo byiza ugereranije na CRS (P <0.05).此外, 与 crs 相比, Sansure ibinyabuzima 检测的阳性率较低 (P <0.05).此外, 与 crs 相比, Sansure ibinyabuzima 检测的阳性率较低 (P <0.05). Кроме того, анализ Sansure biotech имел более уровех ролоних ролоних ролоных рравнен с crs (p <0,05). Byongeye kandi, Sansure bioch isuzuma yari ifite umusaruro wo hasi ugereranije na crs (p <0.05).Sanse Fitech NCAV-2019 (RUO) isesengura rya PPA, NPA na rusange yarenze 93.5% hamwe n'imbaraga za cohen kappa zifite agaciro ka 0.925. Hanyuma, Sansure biotech assay (Ruo) akeneye kurushaho kwemezwa kugirango akoreshwe muri Etiyopiya, kandi ni ubuhe bushakashatsi bugomba gufatwa nko gusuzuma ibirego byabakora.
Igishushanyo mbonera cyo kwiga cyakorewe mu bigo nderabuzima bine i Addis Abeba, ibitaro bya Eka kobe, ikigo cy'itorero ry'ikinyejana cya Zewoditu, n'ibitaro byinzobere mu Mutagatifu Petero. Amakuru yakusanyijwe hagati ya 1 Ukuboza 20 na 3120. Ibikoresho byubuvuzi kuri ubu bushakashatsi byatoranijwe mubyabaye bishingiye ku manza zabo nyinshi ndetse no kuboneka kw'ibigo bikomeye bivurwa mu mujyi. Mu buryo nk'ubwo, ibikoresho, harimo na Aba 7500 na Abbott M2000 mu manota ya PCR, batoranijwe hakurikijwe ibyifuzo by'abakora, kandi ibikoresho bine bya PCR byatoranijwe muri ubu bushakashatsi, nk'uko laboratoire zinyuranye za PCR zatoranijwe muri ubu bushakashatsi, nk'uko laboratoire nyinshi zabajijwe zatoranijwe muri ubu bushakashatsi, nkuko laboratoire nyinshi zabajijwe zatoranijwe muri ubu bushakashatsi, nkuko laboratoire nyinshi zabatabiriye. Ikizamini cya Gene, Abbott SArs-COV-2 Ikizamini cya Sansure, Sars-COV-COV-2 Ikizamini cya BGI cyakozwe mugihe cyo kwiga).
Kwipimisha SARS-COV-2 byakozwe kuva 1 kugeza 30 Ukuboza 2020 ukoresheje ML 3 yo gutwara virusi (VTM) (Ikoranabuhanga ryibitangaza, Shenzhen, Ubushinwa) bwakozwe na Covidi. Ingero za Nasopharyngeal zakusanyirijwe hamwe nabakusanya ibyitegererezo bahuguwe kandi boherezwa muri Efurayi bapakiye triple. Mbere yo kwigunga kwa mucleic, buri cyitegererezo cyahawe nimero idasanzwe. Gukuramo bikorwa kuri buri cyitegererezo ako kanya nyuma yo kuhagera ukoresheje igitabo na Automatic. Rero, kugirango akuremo byikora Abbott M2000, 1.3 ml (harimo 0.8 ml yapfiriyemo icyitegererezo (abbott molecure ya abna ) Icyiciro cya 96 [92 cyicyitegererezo, ubugenzuzi bubiri no kugenzura hamwe nubugenzuzi bubiri (NTC)] byashyizwe mubikorwa rusange (NTC) byashyizwemo muburyo rusange (kugarura no gutahura) kuzenguruka ibiryo-COV-2 mugihe nyacyo. ubucukuzi bw'amabuye y'agaciro. Mu buryo nk'ubwo, kubwo gukuramo intoki, koresha ingero zimwe (kugirango ukuramo byikora no kuvumbura). Rero, byose, 140 byitegererezo byaragaragaye kandi bikuramo ukoresheje Qiamp virusi ya Qna mini kit (qiagen, ubudage, igenzura rya 20 (NTCs ebyiri) hejuru yimitwe icyenda. Intoki zayoherereje amajwi zongerewe kandi zimenyekana na Abi 7500 Ubushyuhe bw'amabuye y'agaciro bakoresheje SARS-COV - 2 BGI Assay, Daan Gene Massay, Daan Gene Massay, na Sansure bilitech asaba.
Kwigunga byikora no kwezwa kwa SARS-COV - 2 virusi ikurikira ihame rya magnetike rikoresha icyitegererezo cya Abbott Abna Gutegura Reage. GUTANGIRA URUGERO NA GUKORA AMAFARANGA YA VILL ikorwa ukoresheje ibikoresho birimo Guanidine Ifite ITHetioriorcyanate kugeza RNAse RNAse. RNA noneho iratandukana na poroteyine nigitandukanya icyiciro gikomeye ukoresheje Silica, ni ukuvuga umunyu wa Guanidinium na alkaline Ph ya lysine buffer iteza imbere aside iricleti kuri Silica (Sio2). Intambwe yo koza ikuraho poroteyine zisigaye kandi imyanda kugirango itange igisubizo gisobanutse. Mucyo RNA yitaruye muri microparticles ishingiye kuri silica bakoresheje imitwe ya rukuruzi wa magneti ya magnetic20,21. Kurundi ruhande, kwigunga mu gitabo no kweza RNA bikorwa muburyo bwa spin bakoresheje centrifugation aho kuba igihagararo cyo gukomera no gutandukanya microCarticles kuva kumuriro.
Ikizamini cya Abbott-Igihe cya SARS-COV-2 cyo kumenya (Inc) cyakozwe hakurikijwe amabwiriza y'abakora, yakiriye EAA199,22 uhereye kuri uwo na FDA. Muri iyi protocole, icyitegererezo cyo gutanga icyitegererezo mbere yo gukuramo cyakozwe mu bwogero bw'amazi kuri 56 ° C saa 30 min. Nyuma yo kudakora virusi, gukuramo acide mucleoke byakorewe kuri abbott M2000 SP igikoresho cyo kuri 0.5 ml vtm ukoresheje abbott M2000 ya ADNAT ya Abnatt. ukurikije uwakoze. Kwiyongera no gutahura byakozwe ukoresheje Abbott M2000 RT-PCR Igikoresho, hamwe no gutahura ibintu byakorewe kuri RDRP na NONE. Rox) na vic p (irangi rya nyirufatiro) kugirango wibande kandi utange igenzura ryimbere, bituma icyarimwe hamenyekane ibicuruzwa byongeweho 19.
Uburyo bwo gutahura uburyo bwo kumenya iyi kikoresho bushingiye kuri intambwe imwe ya RT-PCR. ORF1A / B na N on ont byatoranijwe nkuturere twabitswe na tekinoroji ya Daan Gene kugirango dutangire Akarere ka Appliction. Primes na Fluorescent ikorwa (N gene ikorwa na Fam, Orf1a / B yanditseho vic) yagenewe kumenya SARS-COV-2 RNA. Ivanga ryanyuma na shobuja mibi bateguwe wongeyeho 5 μl yo hejuru kugeza kuri 20 μl ya Shebuja kuvanga ku bwinshi bwa 25 μl. Kwongeza no Gutahura byakozwe icyarimwe kuri ABI 750024 igikoresho-nyacyo cya PCR.
ORF1A / B NA N NEGOs zagaragaye ukoresheje Sansech Biotech NCUV-2019 ICID DIPNOOST ibikoresho (fluorescent pcr gutahura). Tegura ibishobokari byihariye kuri buri ntego gera uhitamo umuyoboro wigihu cya ORF1A / B hamwe numuyoboro wa Rox kuri NE. Kuri iyi Assay Kit, luent na shobuja bavanga reagents yongeyeho: Tegura 30 μl shobuja kuvanga reagent na 20 μL Igihe nyacyo PCR AB 750025 yakoreshejwe mugunyongera / gutahura.
Sars-COV-2 Ikizamini cya BGI ni fluorescent nyayo-igihe cya Rrt-PCR igikoresho cyo gusuzuma Covid-19. Akarere kagenewe kaherereye mukarere ka ORF1A / B ka Sars-COME, nikintu kimwe cya gene ya gene. Byongeye kandi, gene ishinzwe imibereho yubukungu yabantu β-actin ni us ast yagenzuwe imbere. Umwigisha uvanze yateguwe no kuvanga 20 μl ya shebuja kuvanga reagent na 10 μl yicyitegererezo cya RNA yakuweho mu kibaya cyuzuyeho neza. ABI 7500 fluorescent ihagije igikoresho-gisanzwe cya PCR cyakoreshejwe mugunyongera no kumenya. Acide yose ya Acicclic, PCR yiga ibintu kuri buri gisasu, kandi gusobanura ibisubizo byakozwe hakurikijwe amabwiriza yabakozwe (imbonerahamwe ya 3).
Muri iri sesengura igereranya, ntabwo twakoresheje uburyo busanzwe bwo kumenya amasezerano yicyumweru (ibyiza, bibi, kandi muri rusange) nibindi byo kugereranya kubisesengura bine. Buri kizamini kigereranya cyakozwe na CRS, muri ubu bushakashatsi CRS yashyizweho nubutegetsi "icyiza icyo ari cyo cyose" cyagenwe, ntabwo cyarakozwe nikizamini kimwe, twakoresheje byibuze ibisubizo bibiri byikizamini. Byongeye kandi, mugihe cya Covid-19, ibisubizo bibi, ibisubizo bibi ni bibi kuruta ibisubizo byiza. Kubwibyo, kuvuga "ibyiza" neza bishoboka kuva CRRs ibisubizo, byibuze ibizamini bibiri bikora bigomba kuba byiza, bivuze ko byibuze ibisubizo byiza bishoboka ko biva muri EAA iva muri EAA iva muri EAA iva muri EAA iva muri EAA iva muri EAA iva muri EAA iva muri EAA iva muri EAA iva muri EUA iva muri EAUA. Rero, mubisubizo bine byikizamini, ibisubizo bibiri cyangwa byinshi bitanga ibisubizo bimwe bifatwa nkibyiza cyangwa bibi18,27.
Amakuru yakusanyijwe ukoresheje imiterere yamakuru yubatswe, amakuru yinjira hamwe nisesengura byakozwe ukoresheje software yibarurishamibare na SPSS 23.0 kubibare bisobanura. Amasezerano meza, mabi, kandi muri rusange yasesenguwe, kandi amanota ya Kapsa yakoreshejwe mu kumenya urwego rwamasezerano ya buri buryo hamwe na CRS. Indangagaciro za KAPAPA zisobanurwa kuburyo zikurikira: 0.01 kugeza 0.20 kuri amasezerano yoroheje, 0.21 kugeza ku masezerano rusange, 0.41.60 kumasezerano atoroshye, 0.61.90 kuri 0.81.99
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