Gukemura ibibazo bya PCR: Ibibazo bikunze kubazwa nibisubizo

Polymeyese reactions (PCR) isesengura nibikoresho byingenzi muri biologiya bya molecular, bituma abashakashatsi bongera ADN kubisabwa kuva mubushakashatsi bwa genetike hamwe nubushakashatsi bwa kamere. Ariko, kimwe nigikoresho icyo aricyo cyose, umusesengura cya PCR urashobora guhura nibibazo bigira ingaruka kumikorere yayo. Iyi ngingo ikemura ibibazo bimwe na bimwe bijyanyePCR isesenguraGukemura ibibazo no gutanga ibisubizo bifatika kubibazo bisanzwe.

1. Kuki pcr yanjye idasubiza?

Kimwe mu bibazo gikunze kuba abakoresha bahura nabyo ni ibishobora gutuma PCR yifata kugirango yongere intego ya ADN. Ibi birashobora guterwa nibintu byinshi:

Igishushanyo mbonera cya primer kitari cyo: Menya neza ko prirs yawe yihariye ikurikirana kandi ifite ubushyuhe bwiza bwo gushonga (TM). Koresha ibikoresho bya software kuri Premir Igishushanyo kugirango wirinde guhuza.

Inyandikorugero idahagije ADN: Menya neza ko ukoresha icyitegererezo cya ADN. Ntabwo cyane bizavamo intege nke cyangwa ntanyongera.

Ibihimbano murugero: Abanduye murugero barashobora kubuza reaction ya PCR. Tekereza kweza ADN yawe cyangwa ukoresheje uburyo butandukanye bwo gukuramo.

Igisubizo: Reba igishushanyo cyawe cyambere, ongera icyitegererezo cyo kwibandaho, kandi urebe neza ko icyitegererezo cyawe kitarimo ababika.

2. Kuki ibicuruzwa byanjye PCR ifite ubunini butari bwo?

Niba ingano yibicuruzwa bya PCR idateganijwe, irashobora kwerekana ikibazo hamwe nibibazo byo gukora cyangwa ibikoresho byakoreshejwe.

Kwongerera akamaro: Ibi birashobora kubaho niba primer ihambiriye kurubuga rutamenyerewe. Reba umwihariko wa primers ukoresheje igikoresho nko guturika.

Ubushyuhe bukabije: Niba ubushyuhe bukabije ari buke, budasobanutse bushobora gutuma. Guhitamo ubushyuhe bukabije na gradient PCR.

Igisubizo: Emeza umwihariko kandi utezimbere ubushyuhe bukabije kugirango utezimbere ibicuruzwa bya PCR.

3. Isesengura ryanjye rya PCR ryerekana ubutumwa bwibeshya. Nkore iki?

Ubutumwa bwamakosa kusesengura rya PCR burashobora gutera ubwoba, ariko birashobora gutanga ibimenyetso kubibazo bishobora kuba.

Ibibazo bya Calibration: Menya neza ko isesengura rya PCR ryahinduwe neza. Kugenzura buri gihe no kugenzura kalibration ni ngombwa kugirango ubone ibisubizo nyabyo.

Itsinda rya software: Rimwe na rimwe, Amakosa arashobora gutera ibibazo. Ongera utangire mudasobwa yawe hanyuma urebe amakuru agezweho ya software.

Igisubizo: Reba ku gitabo cy'umukoresha kuri kode yihariye hanyuma ukurikize intambwe zasabwe. Kubungabunga buri gihe birashobora gukumira ibibazo byinshi.

4. Kuki PCR yakiriye ibisubizo bidahuye?

Ibisubizo bya PCR bidahuye birashobora kubabaza kubwimpamvu nyinshi:

Ubwiza bwa Reagent: Menya neza ko reagent zose, harimo imisemburo, buffers, na drtps, ni shyashya kandi ryiza. Byarangiye cyangwa byanduye byanduye bishobora gutera guhinduka.

Ubushyuhe bwa Cycler Calibration: Igenamiterere ryubushyuhe ntirishobora kugira ingaruka kubikorwa bya PCR. Guhora ugenzure kalibration ya cycler yubushyuhe.

Igisubizo: Koresha uburyo bwiza bwo gufata amajwi no guhindura urubuga rwawe rwubushyuhe buri gihe kugirango umenye ibisubizo bihamye.

5. Nigute ushobora kunoza imikorere ya PCR gukora neza?

Kunoza imikorere ya PCR birashobora kuganisha kumusaruro mwinshi nibindi bisubizo byizewe.

Kunoza uko ibintu byahinduwe: Igeragezwa ukoresheje ibitekerezo bitandukanye bya Primers, inyandikorugero ya ADN na MGCL2. Buri pcr reaction irashobora gusaba ibintu bidasanzwe kubikorwa byiza.

Koresha extmes ndende: Niba ari ukuri kunegura, tekereza ukoresheje ubudahemuka bwa ADN hejuru ya ADN kugirango ugabanye amakosa mugihe cyongerewe.

Igisubizo: Kora ubushakashatsi bwo kumenya kugirango ubone ibihe byiza kuri PCR yihariye PCR.

Muri make

Gukemura ikibazo aPCR isesenguraIrashobora kuba umurimo utoroshye, ariko usobanukirwe ibibazo bisanzwe nibisubizo byabyo birashobora kuzamura cyane uburambe bwa PCR. Mugukemura ibibazo bisanzwe, abashakashatsi barashobora kunoza ibisubizo bya PCR kandi bakize ibisubizo byizewe muri porogaramu ya Molecular. Kubungabunga buri gihe, Guhitamo Witonze Ibishishwa, no Gutezimbere Ibisabwa Byifashe ni Urufunguzo rwo gusesengura isesengura rya PCR.