Amakuru - Fungura umupfundikizo hanyuma urebe - Big Fish 40mins indwara yingurube ibisubizo byihuse

Fungura umupfundikizo hanyuma urebe - Big Fish 40mins indwara yingurube byihuse

Indwara nshya yingurube ikonjesha-yumisha reagent ya Big Fish yatangijwe. Bitandukanye na reagent ya gakondo isaba gutegurwa nintoki za sisitemu yo kubyitwaramo, iyi reagent ifata imiterere ya microse yumubumbe wuzuye ivanze, ishobora kubikwa mubushyuhe bwicyumba bitagize ingaruka kumikorere ya reagent. Mugihe cyo gutahura, acide nucleic yakuwe gusa igomba kongerwaho mugukingura umupfundikizo. Reagent imaze gushonga burundu, irashobora kugeragezwa kumashini. Big Fish ifatanije na reagent ya nucleic acide yuzuye hamwe nibikoresho bikomoka kuri Big Fish, Big Fish yatangije kumugaragaro igisubizo cyiminota 40 yo gutahura vuba indwara zingurube. Hamwe nimishinga itandatu yingenzi yo gutahura harimo ugutwi kwubururu, Pseudorabies, Indwara yingurube, Circovirus, Non Circovirus, na ibicurane bya Porcine, bifata iminota igera kuri 40 kugirango urangize inzira yose ya PCR fluorescence yo kubara uhereye kubitunganyirize kugeza kubisubizo.

Inzira yo gukemura

1. Gukuramo neza - ingero nyinshi zirashobora gutunganywa muminota 10
Ukoresheje Big Fish kwisi yose ya acide nucleic acide hamwe nogusukura reagent ihujwe hamwe nogukuramo acide nucleique yuzuye kandi ikanabisukura, gukuramo aside nucleic birashobora gukorerwa kuburugero rutandukanye (harimo amaraso yose, serumu, plasma, swabs yibidukikije, swabs yo munwa, fecal swabs, nibindi) mugihe cyiminota 10 bitabaye ngombwa ko umuntu yitegura mbere. Nyuma yo gupakira icyitegererezo, irashobora gukururwa kuri mashini.

2. Kwiyongera byihuse -30 min byihuta bya fluorescence
Ukoresheje Ifi Nini BFOP-1650 Fluorescence Quantitative PCR Analyser, uburyo bwo mwishyamba bwa Big Fish freeze yumye yumye reagent irashobora gukora. Ihuriro rya reagent yo gukonjesha hamwe na gahunda yiminota 30 yihuse irashobora kugera kumupfundikizo no kwipimisha kurubuga.

3. Isesengura ryubwenge - imikorere yingenzi, isesengura ryikora
Isesengura rinini rya BFOP-1650 fluorescence yuzuye PCR isesengura ntabwo isaba igenamigambi rigoye. Kanda "Tangira" kubintu byose byerekana kugirango utangire gutahura. Nyuma ya amplification irangiye, ihita ikora urubanza rwiza kandi rubi nta gusesengura amakuru yintoki.

Igihe cyo kohereza: Jun-21-2025