Akamaro ka Calibration kumikorere ya Thermal Cycler Performance

Amagare yubushyuhenibikoresho byingirakamaro mubijyanye na biologiya biologiya nubushakashatsi bwa genetics. Bikunze kwitwa imashini ya PCR (polymerase chain reaction), ibi bikoresho nibyingenzi mugukomeza ADN ikurikirana, bituma abahanga bakora ubushakashatsi butandukanye kuva clon kugeza isesengura rya gene. Nyamara, imikorere yumukino wamagare ushingiye cyane kuri kalibrasi yayo, bityo abashakashatsi bagomba kumva akamaro kiki gikorwa.

Calibration ni inzira yo guhindura no kugenzura ukuri kw'ibipimo by'ibikoresho ukurikije ibipimo bizwi. Kuri cycle yumuriro, ibi bikubiyemo kwemeza ko imiterere yubushyuhe itomoye kandi ihamye mugihe cyamagare. Ukuri mukugenzura ubushyuhe nibyingenzi, kuko no gutandukana guto birashobora gutuma habaho itandukaniro rikomeye mubisubizo byubushakashatsi bwa PCR. Kurugero, niba ubushyuhe bwo gutandukana butagerwaho, imirongo ya ADN ntishobora gutandukana neza, bikavamo amplification idakora neza. Mu buryo nk'ubwo, niba ubushyuhe bwa annealing buri hasi cyane cyangwa hejuru cyane, birashobora gutuma habaho guhuza bidafite akamaro cyangwa kubura burundu guhuza, amaherezo bikabangamira ubusugire bwubushakashatsi.

Imwe mumpamvu nyamukuru zituma kalibrasi ari ingenzi kubasiganwa ku magare yubushyuhe ningaruka igira ku myororokere. Mubushakashatsi bwa siyanse, kubyara ni ishingiro ryizerwa. Niba umukinyi w'amashanyarazi adahinduwe neza, ibisubizo byabonetse mubushakashatsi butandukanye birashobora gutandukana, bikagora kwigana ibyavuye mubushakashatsi. Uku kudahuza kurashobora kuganisha ku myanzuro itari yo no gutakaza umutungo, bikabangamira agaciro k'ubushakashatsi. Calibration isanzwe yemeza ko cycler yumuriro ikora mubipimo byagenwe, bityo bikongerera ubwizerwe bwibisubizo byawe.

Ikigeretse kuri ibyo, akamaro ka kalibrasi ntikuri gusa muburyo bwo kumenya ubushyuhe, ahubwo no muburyo bumwe bwo gukwirakwiza ubushyuhe muri cycle yumuriro. Igikoresho cyateganijwe neza kigomba kuba gishobora gutanga ubushyuhe buhoraho kumariba yose mumasahani menshi. Ubushyuhe butandukanye burashobora gutuma habaho itandukaniro mubipimo byiyongera, bishobora kugira ingaruka kubisubizo hanyuma amaherezo y'ibisubizo rusange byubushakashatsi. Mugihe cyo guhinduranya ubushyuhe bwumuriro, abashakashatsi barashobora kwemeza ko ingero zose ziri mubihe bimwe byubushyuhe, bityo bikazamura ireme ryamakuru.

Usibye kunonosora neza no gusubiramo, guhora uhinduranya cycler yawe yumuriro birashobora kongera ubuzima bwibikoresho. Igihe kirenze, ibice biri mumashanyarazi yumuriro birashobora gushira cyangwa bigahinduka bike, biganisha kumakosa ashobora kuba. Muguhindura ibikoresho buri gihe, abashakashatsi barashobora kumenya no gukemura ibibazo mbere yuko biba ibibazo bikomeye, bakemeza ko umukinnyi wamagare yumuriro aguma mubikorwa byiza. Ubu buryo bukora kuri kalibrasi ntabwo bubika gusa ibiciro bijyanye no gusana cyangwa gusimburwa, ariko kandi bigabanya igihe cyo gukora muri laboratoire.

Muri make, kalibrasi yaAmagare yubushyuheni ikintu cyibanze cyo kwemeza imikorere yabo no kwizerwa mubushakashatsi bwa siyansi. Kugenzura ubushyuhe bwuzuye hamwe nuburinganire nibyingenzi kugirango intsinzi ya PCR nubundi bushakashatsi bushingiye ku bushyuhe. Mugukora kalibrasi isanzwe, abashakashatsi barashobora kunoza imyororokere y ibisubizo, kugumana ubusugire bwibyo babonye, ​​no kongera ubuzima bwibikoresho byabo. Mugihe urwego rwibinyabuzima rwa molekuline rukomeje gutera imbere, akamaro ka kalibrasi yumuriro wa cycle izakomeza kuba ikintu cyingenzi muguteza imbere siyanse no guhanga udushya.