Nigute ushobora guhitamo icyerekezo cyumuriro gikenewe kubushakashatsi bwawe

Amagare yubushyuheni ibikoresho by'ingirakamaro iyo bigeze ku binyabuzima bya molekuline n'ubushakashatsi bwa geneti. Bizwi kandi nka mashini ya PCR (polymerase chain reaction), iki gikoresho ningirakamaro mugukomeza ADN, bigatuma iba umusingi wibikorwa bitandukanye birimo cloni, ikurikiranye hamwe nisesengura rya gene. Nyamara, hari amahitamo menshi kumasoko kuburyo guhitamo icyuma gikwirakwiza ubushyuhe bwumuriro kubyo ukeneye ubushakashatsi birashobora kuba umurimo utoroshye. Hano hari ibintu by'ingenzi ugomba gusuzuma mugihe uhisemo.

1. Sobanukirwa n'ibisabwa ubushakashatsi

Mbere yo kwibira mubisobanuro byamagare atandukanye yubushyuhe, ni ngombwa gusuzuma ubushakashatsi bwawe bukenewe. Reba ubwoko bw'igerageza uzakora. Urimo ukoresha PCR isanzwe, PCR yuzuye (qPCR), cyangwa progaramu-yinjiza cyane? Buri kimwe muri ibyo bisabwa gishobora gusaba ibintu bitandukanye nubushobozi bwumukino wamagare.

2. Ubushyuhe Urwego hamwe nuburinganire

Ubushyuhe buringaniye bwa cycle yumuriro ni ikintu gikomeye. Porotokole nyinshi za PCR zisaba intambwe yo gutandukana hafi ya 94-98 ° C, intambwe ya annealing kuri 50-65 ° C, nintambwe yo kwaguka kuri 72 ° C. Menya neza ko ubushyuhe bwumuriro wahisemo bushobora gukoresha ubu bushyuhe kandi ko ubushyuhe bwagabanijwe neza muri module. Ubushyuhe buke burashobora kugira ingaruka kubushakashatsi bwawe butera ibisubizo bidahuye.

3. Hagarika imiterere n'ubushobozi

Abatwara amagare yubushyuhe baza muburyo butandukanye bwa modular, harimo amasahani 96-meza, amasahani 384, ndetse nibisahani 1536. Guhitamo imiterere yo guhagarika bigomba guhuza ibyo ukeneye. Niba ukora ubushakashatsi bwimbitse, ushobora gukenera imiterere nini yo guhagarika. Ibinyuranye, kubushakashatsi buto, isahani 96-iriba irashobora kuba ihagije. Byongeye kandi, tekereza niba ukeneye guhinduranya muburyo butandukanye, kuko ibi bishobora kongera ubumenyi bwubushakashatsi bwawe.

4. Umuvuduko no gukora neza

Muri iki gihe ubushakashatsi bwihuse bwibidukikije, igihe nicyo kintu. Shakisha icyuma gishyuha gifite ubushyuhe bwihuse no gukonjesha. Moderi zimwe zateye imbere zirashobora kuzenguruka PCR muminota 30, byihutisha akazi kawe. Mubyongeyeho, ibintu nkuburyo bwihuse cyangwa ubushyuhe bwihuse bwongera imikorere, bikwemerera gutunganya ibyitegererezo byinshi mugihe gito.

5. Umukoresha Imigaragarire na software

Imigaragarire yumukoresha ni ngombwa kugirango ikore neza. Shakisha icyuma gishyushya ubushyuhe hamwe na ecran yo gukoraho, uburyo bworoshye bwo gutangiza porogaramu, hamwe na protocole. Moderi igezweho irashobora kandi kuza hamwe na software yemerera kugenzura-igihe no gusesengura amakuru, bifitiye akamaro kanini porogaramu za qPCR. Menya neza ko software ijyanye na sisitemu zisanzweho kandi irashobora gukemura amakuru ukeneye.

6. Ibitekerezo

Amagare yubushyuhe aratandukanye cyane kubiciro, nibyingenzi rero kugira bije mbere yuko utangira kugura imwe. Mugihe bishobora kuba bigerageza kujyana nuburyo buhendutse, tekereza ku gihe kirekire cyo gushora imari mumashini yujuje ubuziranenge yujuje ibyifuzo byawe. Ntuzirikane gusa igiciro cyambere cyo kugura, ahubwo urebe nigiciro cyibikoreshwa, kubungabunga, hamwe nibishobora kuzamurwa.

7. Inkunga yinganda na garanti

Hanyuma, tekereza urwego rwinkunga na garanti yatanzwe nuwabikoze. Umukinnyi wamagare wizewe agomba gutanga garanti yuzuye kandi akagira ubufasha bwabakiriya mugukemura ibibazo no kubungabunga. Ibi bigutwara umwanya numutungo mugihe kirekire.

mu gusoza

Guhitamo uburenganziraumukerarugendokubushakashatsi bwawe bukenewe nicyemezo gikomeye gishobora kugira ingaruka kubigeragezo byawe. Urebye witonze ibisabwa byihariye, urwego rwubushyuhe, imiterere ya module, umuvuduko, interineti yumukoresha, ingengo yimishinga, hamwe ninkunga yinganda, urashobora guhitamo neza bizamura ubushobozi bwubushakashatsi bwawe kandi ubone ibisubizo byizewe. Gushora umwanya muriki gikorwa cyo gutoranya amaherezo bizatanga umusaruro mubikorwa byiza bya siyansi.