Nigute wahitamo uburyo bwiza bwumuyaga kubushakashatsi bwawe

Cyclenibikoresho byimpapuro iyo bigeze kuri ibinyabuzima bya molecular hamwe nubushakashatsi bwa genetike. Bizwi kandi nka PCR (imashini ya Polymeyese), iki gikoresho ningirakamaro muguhaza ADNA, bituma ikomeza imfuruka yibisabwa bitandukanye birimo cloning, isesengura rikurikirana kandi rikurikirana. Ariko, hariho amahitamo menshi kumasoko ahitamo ikirere cyiza cyubushyuhe kubushakashatsi bwawe burashobora kuba umurimo utoroshye. Hano hari ibintu bimwe byingenzi ugomba gusuzuma mugihe wahisemo.

1. Sobanukirwa n'ibisabwa

Mbere yo kwibira mubisobanuro byinyoni zitandukanye zubushyuhe, ni ngombwa gusuzuma ibyo ukeneye ubushakashatsi. Reba ubwoko bw'ikigereranyo uzakora. Urimo ukoresha PCR isanzwe, PCR PCR (QPCR), cyangwa porogaramu yo hejuru? Buri kimwe muribi porogaramu gishobora gusaba ibintu bitandukanye nubushobozi bwumuyaga.

2. Ubushyuhe butarenze

Ubushyuhe bwa cycler yubushyuhe nikintu gikomeye. Amasezerano menshi ya PCR asaba intambwe yo kwamagana 94-98 ° C, Intambwe yo Gutemba kuri 50-65 ° C, nintambwe yo kwagura saa moya za 72 ° C. Menya neza ko ikirere cyiza wahisemo gishobora gukora ubu bushyuhe kandi ko ubushyuhe butangwa no muri module. Ubushyuhe buke bukabije burashobora kugira ingaruka mubushakashatsi bwawe batera ibisubizo bidahuye.

3. Guhagarika imiterere nubushobozi

Ubushyuhe bw'imishuro butera mu miterere itandukanye, harimo amasahani 96, amasaha y'imyaka 384, ndetse n'isahani y'ibyamamare. Guhitamo imiterere yahagaritswe bigomba guhuza ibikenewe byawe. Niba ukora ubushakashatsi bwinyungu buke, urashobora gukenera imiterere nini yo guhagarika. Ibinyuranye, kubigeragezo bito, isahani 96-igorofa irashobora kuba ihagije. Byongeye kandi, tekereza niba ukeneye module yo guhumeka muburyo butandukanye, kuko ibi birashobora kongera uburyo bwubushakashatsi bwawe.

4. Umuvuduko no gukora neza

Mubihe byuyu munsi byubushakashatsi bwihuse, igihe nicyo cyingenzi. Shakisha ukwezi kwubushyuhe hamwe nubushobozi bwo gushyuha no gukonjesha. Imyaburo imwe yateye imbere irashobora kuzuza uruziga rwa PCR muminota 30, kwihutisha cyane akazi kawe. Byongeye kandi, ibiranga uburyo bwihuse cyangwa umubare wubushyuhe bwihuse wongera imikorere, kukwemerera gutunganya ingero nyinshi mugihe gito.

5. Umukoresha na software

Umukoresha-winshuti ni ngombwa mugukora neza. Shakisha uruhabumbe rwumuriro hamwe na ecran ya matgutive, uburyo bworoshye bwo gutangiza porogaramu, na pretocole. Moderi Yambere irashobora kandi kuza hamwe na software yemerera igihe cyo gukurikirana no gusesengura amakuru nyayo, ingirakamaro cyane cyane kubisaba QPCR. Menya neza ko software ihujwe na sisitemu yawe ihari kandi irashobora gukemura ibibazo byo gusohoka amakuru ukeneye.

6. Ibitekerezo by'ingengo y'imari

Ubushyuhe bwubushyuhe buratandukanye cyane kubiciro, ni ngombwa rero kugira ingengo yimari mbere yuko utangira kugura imwe. Nubwo bishobora kuba bigerageza kujyana nuburyo buhendutse, suzuma agaciro k'igihe kirekire cyo gushora imari mu mashini nziza yujuje ibikenewe byubushakashatsi. Ntimutekereze gusa igiciro cyambere cyo kugura, ahubwo gisuzumwa nigiciro cyakoreshwa, kubungabunga, no kuzamura.

7. Inkunga y'abakora na garanti

Hanyuma, tekereza kurwego rwinkunga na garanti yatanzwe nuwabikoze. Ubushyuhe bwizewe bugomba gutanga garanti yuzuye kandi ifite inkunga y'abakiriya yo gukemura ibibazo no kubungabunga. Ibi bigukiza umwanya numutungo mugihe kirekire.

Mu gusoza

Guhitamo uburenganziraUbushyuhe ByclerErega ibikenewe byubushakashatsi nicyemezo gikomeye gishobora kugira icyo ugeraho. Mugusuzuma witonze ibisabwa byihariye, ubushyuhe, imiterere ya module, umuvuduko, imikoreshereze yumukoresha, hamwe niterambere ryubushobozi buzamura ubushobozi bwubushakashatsi kandi ubone ibisubizo byizewe. Gushora igihe muriyi nzira yo gutoranya amaherezo bizahita byishura muburyo bwiza no gukora akazi ka siyansi.