Ubutaka, nkibidukikije bitandukanye, bukungahaye kuri mikorobe, harimo ubwoko butandukanye bwa mikorobe nka bagiteri, fungi, virusi, cyanobacteria, actinomycetes, protozoa na nematode. Gutunga ibikorwa byinshi bya metabolike hamwe na physiologique, bigira uruhare runini mukuzunguruka intungamubiri zubutaka kandi ni ngombwa mugukuraho ubutaka bwanduye. Nka kimwe mu bidukikije bitandukanye ku isi, ubushakashatsi bwibinyabuzima bwa molekuline bwubutaka bifite akamaro gakomeye k’ibinyabuzima. Muri ubu buryo, kubona ADN ya mikorobe ikomoka kubutaka nintambwe yambere mubushakashatsi bwubutaka nintambwe ikomeye cyane kugirango intsinzi yubushakashatsi bwimbere. Nyamara, usibye umutungo wa mikorobe ikungahaye, ubutaka bukunze kuba burimo metabolite nyinshi (acide humic, aside xanthic nibindi bintu bya humic), bishobora kwezwa byoroshye hamwe na acide nucleic mugihe cyo gukuramo aside nucleic, bikagira ingaruka kumasoko ya PCR no muburyo bukurikirana.Ifi niniGukurikirana Ubutaka hamwe na Faecal Genomic ADN yo gutunganya ibikoresho birashobora gukuramo neza kandi byihuse ADN ya genomique yera kandi yibanda cyane kuri sample ikungahaye kuri humus nkimyanda yubutaka, ikaba ifasha cyane mubutaka bwa mikorobe yibinyabuzima bitandukanye.
Ibicuruzwa binini by'amafi
Igicuruzwa gikoresha sisitemu yihariye idasanzwe yatejwe imbere kandi itezimbere hamwe na magnetiki amasaro ahuza cyane cyane ADN, ishobora guhuza byihuse na adsorb, gutandukanya no kweza acide nucleic, bigatuma biba byiza mu bwigunge bwihuse kandi bunoze no kweza ADN genomic mu butaka n’umwanda, ndetse no kuvanaho ibisigazwa nka acide humic, proteyine, ion yumunyu, nibindi bisigazwa. Guhuza na Beaglefly ikurikiranye uburyo bwa magnetiki isaro uburyo bwa nucleic aside ikuramo, birakwiriye cyane kuvana mu buryo bwikora ubunini bunini bw'icyitegererezo. ADN yakuwe muri ADN ifite isuku nini kandi nziza kandi irashobora gukoreshwa cyane muri PCR / qPCR, NGS nubundi bushakashatsi bwubushakashatsi.
Ibiranga
Ubwiza bwiza:kwigunga no kweza ADN genomic, umusaruro mwinshi, ubuziranenge bwiza.
Ingero nini z'icyitegererezo:irashobora gukoreshwa cyane mubwoko bwose bwubutaka hamwe nicyitegererezo.
Byihuse kandi byoroshye:Gukuramo byikora hamwe nibisohoka bihuye, cyane cyane bikwiranye no gukuramo ingano nini yintangarugero.
Umutekano kandi udafite uburozi:ntagikeneye uburozi bwa organic reagent nka phenol / chloroform, nibindi.
Ibikoresho bihuza n'imiterere:BFEX-32 / BFEX-32E / BFEX-96E
Igihe cyo kohereza: Nyakanga-10-2025