Kwipimisha methylation ya ADN byahujwe na terefone zigendanwa hakiri kare gusuzuma ibibyimba no gusuzuma leukemia hamwe nukuri kuri 90.0%!

Kumenya hakiri kare kanseri ishingiye kuri biopsy y'amazi ni icyerekezo gishya cyo kumenya kanseri no gusuzuma indwara yatanzwe n'ikigo cy'igihugu cya kanseri cyo muri Amerika mu myaka yashize, hagamijwe kumenya kanseri hakiri kare cyangwa se ibikomere byabanjirije. Yakoreshejwe cyane nka biomarker nshyashya mugupima hakiri kare indwara mbi zitandukanye, harimo kanseri y'ibihaha, ibibyimba byo mu gifu, glioma n'ibibyimba by'abagore.

Kugaragara kwa platifomu kugirango tumenye imiterere ya methylation (Methylscape) biomarkers ifite ubushobozi bwo kunoza cyane kwipimisha hakiri kare kanseri, bigashyira abarwayi mugihe cyambere cyo kuvurwa.

Vuba aha, abashakashatsi bakoze uburyo bworoshye kandi butaziguye bwo kumenya imiterere ya methylation yo gutahura bishingiye kuri cysteamine itatse zahabu ya nanoparticles (Cyst / AuNPs) ihujwe na biosensor ishingiye kuri terefone ituma hasuzumwa vuba hakiri kare ibibyimba byinshi. Kwipimisha hakiri kare kuri leukemia birashobora gukorwa mugihe cyiminota 15 nyuma yo gukuramo ADN mu cyitegererezo cyamaraso, hamwe na 90.0%. Umutwe w'ingingo ni Kumenya vuba ADN ya kanseri mu maraso y'abantu ukoresheje AuNPs ya sisitemu na sisitemu yo kwiga imashini。

Igicapo 1. Urubuga rworoshye kandi rwihuse rwo gusuzuma kanseri hifashishijwe ibice bya Cyst / AuNPs birashobora gukorwa muburyo bubiri bworoshye.

Ibi birerekanwa mu gishushanyo cya 1. Icya mbere, igisubizo cyamazi cyakoreshejwe mu gushonga ibice bya ADN. Cyst / AuNPs noneho yongewe kumuti uvanze. ADN isanzwe kandi mbi ifite imiterere ya methylation itandukanye, bikavamo ibice bya ADN hamwe nuburyo butandukanye bwo kwiteranya. Ubusanzwe ADN yegeranya bidatinze kandi amaherezo ikusanya Cyst / AuNPs, bikavamo imiterere itukura ya Cyst / AuNPs, kugirango ihinduka ryibara riva mumutuku rijye mubururu rishobora kugaragara n'amaso. Ibinyuranye, imiterere yihariye ya methylation ya kanseri ADN itera kubyara amatsinda manini y'ibice bya ADN.

Amashusho yibyapa 96 byafashwe hakoreshejwe kamera ya terefone. ADN ya kanseri yapimwe na terefone ifite ibikoresho byo kwiga imashini ugereranije nuburyo bushingiye kuri spekitroscopi.

Kwipimisha kanseri mubyitegererezo byamaraso

Kugirango bagure akamaro k'urubuga rwa sensing, abashakashatsi bakoresheje sensor itandukanya neza ADN isanzwe na kanseri mubyitegererezo by'amaraso nyayo. methylation ishusho kurubuga rwa CpG igenga imiterere ya gene. Mu bwoko bwa kanseri hafi ya bwose, impinduka za methylation ya ADN bityo rero mukugaragaza genes zitera tumourigenezi byagaragaye ko zisimburana.

Nkicyitegererezo cyizindi kanseri zifitanye isano na methylation ya ADN, abashakashatsi bifashishije urugero rwamaraso y’abarwayi ba leukemia ndetse n’ubugenzuzi bwiza kugira ngo bakore iperereza ku miterere y’imiterere ya methylation mu gutandukanya kanseri ya kanseri. Iyi methylation landcape biomarker ntabwo iruta gusa uburyo bwihuse bwo gusuzuma leukemia yihuse, ariko kandi irerekana uburyo bushoboka bwo kwaguka hakiri kare kumenya kanseri zitandukanye ukoresheje ubu buryo bworoshye kandi bworoshye.

Hasesenguwe ADN yavuye mu maraso y’abarwayi 31 ba leukemia n’abantu 12 bafite ubuzima bwiza. nkuko bigaragara mu gasanduku kari mu gishushanyo cya 2a, ugereranije no kwinjiza kanseri ya kanseri (ΔA650 / 525) yari munsi ya ADN uhereye ku ngero zisanzwe. ibi byatewe ahanini na hydrophobicity yongerewe imbaraga biganisha ku gukusanya cyane ADN ya kanseri, yabuzaga kwegeranya Cyst / AuNPs. Kubera iyo mpamvu, iyi nanoparticles yakwirakwijwe rwose mubice byo hanze bya kanseri, bituma habaho gutandukana gutandukanye kwa Cyst / AuNPs byamamajwe kuri ADN isanzwe na kanseri. Imirongo ya ROC noneho yakozwe muguhindura imbibi kuva ku giciro gito cya ΔA650 / 525 kugeza ku giciro kinini.

Igishushanyo 2. (a) Indangagaciro zifatika zifatika za cyst / AuNPs zerekana ko hariho dna isanzwe (ubururu) na kanseri (umutuku) dna mubihe byiza.

(DA650 / 525) by'amasanduku; (b) Isesengura ROC no gusuzuma ibizamini byo gusuzuma. (c) Matrix yo kwitiranya gusuzuma abarwayi basanzwe na kanseri. (d) Ibyiyumvo, umwihariko, agaciro keza ko guhanura (PPV), agaciro keza ko guhanura (NPV) hamwe nukuri kuburyo bwateye imbere.

Nkuko bigaragara ku gishushanyo cya 2b, agace kari munsi yu murongo wa ROC (AUC = 0.9274) wabonye kuri sensor yateye imbere yerekanaga sensibilité nini kandi yihariye. Nkuko bigaragara mubisanduku, ingingo yo hasi yerekana itsinda rya ADN isanzwe ntabwo itandukanijwe neza nu mwanya muremure uhagarariye itsinda rya ADN ya kanseri; kubwibyo, gusubira inyuma kwakoreshejwe gutandukanya amatsinda asanzwe na kanseri. Urebye urutonde rwibihinduka byigenga, rugereranya amahirwe yo kuba ikintu kibaho, nka kanseri cyangwa itsinda risanzwe. Impinduka zishingiye ku ntera iri hagati ya 0 na 1. Ibisubizo rero birashoboka. Twagaragaje amahirwe yo kumenya kanseri (P) dushingiye kuri ΔA650 / 525 kuburyo bukurikira.

aho b = 5.3533, w1 = -6.965. Kubisobanuro by'icyitegererezo, amahirwe yo kuba munsi ya 0.5 yerekana icyitegererezo gisanzwe, mugihe bishoboka ko 0.5 cyangwa irenga byerekana urugero rwa kanseri. Igishushanyo 2c cyerekana matrix yo kwitiranya ibintu yavuye mu kiruhuko-cyonyine cyambukiranya imipaka, cyakoreshejwe mu kwemeza ituze ryuburyo bwo gutondeka. Igishushanyo cya 2d cyerekana incamake yo gusuzuma ikizamini cyo gusuzuma uburyo, harimo sensibilité, umwihariko, agaciro keza ko guhanura (PPV) nagaciro keza ko guhanura (NPV).

Biosensor ishingiye kuri terefone

Kugirango barusheho koroshya ibizamini by'icyitegererezo badakoresheje spekitifotometero, abashakashatsi bakoresheje ubwenge bwa artile (AI) kugirango basobanure ibara ry'umuti kandi batandukane kubantu basanzwe na kanseri. Urebye ibi, iyerekwa rya mudasobwa ryakoreshejwe muguhindura ibara ryumuti wa Cyst / AuNPs muri ADN isanzwe (ibara ry'umuyugubwe) cyangwa ADN ya kanseri (umutuku) ukoresheje amashusho yibisahani 96 byafashwe na kamera ya terefone igendanwa. Ubwenge bwa gihanga burashobora kugabanya ibiciro no kunoza uburyo bwo gusobanura ibara ryibisubizo bya nanoparticle, kandi udakoresheje ibikoresho bya optique bya optique. Hanyuma, moderi ebyiri zo kwiga imashini, zirimo Ishyamba rya Random (RF) hamwe na Machine Vector Machine (SVM) bahuguwe kubaka icyitegererezo. moderi zombi za RF na SVM zashyize muburyo bwiza ingero nkibyiza nibibi hamwe na 90.0%. Ibi birerekana ko gukoresha ubwenge bwubuhanga muri terefone igendanwa bishingiye kuri biosensing birashoboka rwose.

Igishushanyo 3. (a) Intego yicyiciro cyibisubizo byanditswe mugihe cyo gutegura icyitegererezo cyintambwe yo gushaka amashusho. (b) Urugero ntangarugero rwafashwe mugihe cyo gushaka amashusho. .

Bakoresheje Cyst / AuNPs, abashakashatsi bakoze neza uburyo bworoshye bwo kwiyumvisha imiterere ya methylation hamwe na sensor ishobora gutandukanya ADN isanzwe na ADN ya kanseri mugihe bakoresheje urugero rwamaraso nyayo mugupima leukemia. Rukuruzi rwakozwe rwerekanye ko ADN yakuwe mu maraso nyayo yashoboye kumenya vuba kandi bidahenze kumenya umubare muto wa kanseri ya ADN (3nM) ku barwayi ba leukemia mu minota 15, kandi ikerekana ko ari 95.3%. Kugirango tworoshe kwipimisha icyitegererezo mugukuraho ibikenewe kuri spekitifotometero, kwiga imashini byakoreshejwe mugusobanura ibara ryigisubizo no gutandukanya abantu basanzwe na kanseri bakoresheje ifoto ya terefone igendanwa, kandi ukuri kwarashoboye kugerwaho kuri 90.0%.

Reba: DOI: 10.1039 / d2ra05725e