Kwipimisha kwa ADNA hamwe na terefone ya terefone kugirango ugaragaze hakiri kare ibibyimba na leukemia kugenzura neza 90.0%!

Gutahura kare kwa kanseri hashingiwe ku biopsy y'amazi ni icyerekezo gishya cyo kumenya kanseri no gusuzuma ibizamini bya kanseri yo muri Amerika mu myaka yashize, hagamijwe gutahura kanseri hakiri kare cyangwa ibisebe byintege nke. Byakoreshejwe cyane nka kilimari ya Novel mu gusuzuma hakiri kare, harimo na kanseri y'ibihaha, ibibyimba byo mu bihaha, ibibyimba byo mu gatsiko, Gliomas hamwe na Glioma na Dumeri.

Hagaragaye platform kugirango umenye imiterere yubusa (MethysCape) ifite ubushobozi bwo kunoza uburyo bwo gusuzuma bwa kanseri, ashyira abarwayi mugihe cyambere cyubwidozi bwambere.

Vuba aha, abashakashatsi bateje imbere urubuga rworoshye kandi ruvuga ko hatanura imiterere yamashanyarazi hashingiwe kuri cystaamine zahabu nanoparticles zahabu nanoparceurced ya ecranphone ifasha kwihuta kwibigera mubyiciro byinshi. Gusuzuma hakiri kare bya leukemia birashobora gukorwa mugihe cyiminota 15 nyuma ya ADN uhereye kumurongo wamaraso, hamwe nukuri 90.0%. Umutwe w'ingingo ni ugutahura kanseri ADN mu maraso y'abantu ukoresheje igituba-capps-capps-imashini yo kwiga imashini.

Igishushanyo 1. Ihuriro ryoroshye kandi ryihuta ryo gusuzuma kanseri ukoresheje Cyst / Ibice bigize Gutanga birashobora kugerwaho mu ntambwe ebyiri zoroshye.

Ibi birerekanwa ku gishushanyo cya 1. Ubwa mbere, igisubizo cy'amazebwe cyakoreshejwe mu gushonga ibice bya ADN. Cyst / Aduns yongeyeho igisubizo kivanze. ADN isanzwe kandi mbi ifite imitungo itandukanye, bikaviramo ibice bya ADN hamwe nuburyo butandukanye bwo kwiteranya. Ubusanzwe ADGGUBAGAYE CYANE KANDI IYAMAGARA CYANE / ANGPS, bikavamo kamere itukura ya Cyst / anps, kugirango impinduka ziva mu ibara ry'umutuku zirashobora kubahirizwa n'ijisho ry'umutuku. Ibinyuranye, umwirondoro wihariye wa kanseri Amna ugana kumusaruro w'amatsinda manini ya ADN.

Amashusho yisahani 96-yerekana neza ukoresheje kamera ya terefone. Kanseri ADN yapimwe na terefone ifite imashini yiga ugereranije nuburyo bushingiye ku buryo.

Gusuzuma kanseri mubyitegererezo byamaraso nyayo

Kugira ngo abaperereza bihangane, abashakashatsi bakoresheje sensor batandukanijwe neza hagati ya ADN isanzwe na kanseri mubyitegererezo byamaraso nyayo. Ubushuhe kuri CPG ku mbuga za CPG epigene bugenga imvugo ya gene. Mugihe kimwe cya kabiri cya kanseri, impinduka muri Methylative ya ADN bityo zigaragaza ingirabuzimafatizo ziteza imbere tumourigenessiis zagaragaye kugirango zisimbukire.

Nk'icyitegererezo ku zindi kanseri zijyanye n'imyigaragambyo ya ADN, abashakashatsi bakoresheje amaraso y'abarwayi ba Leukemia n'ubutegetsi bwiza bwo gukora iperereza ku miterere y'ibibanza by'ibishanga mu gutandukanya kanseri ya Leukaemic muri Sixkaemic. Ikinyabuzima cyibice byahormate ntabwo ari hanze uburyo bwo gusuzuma byihuse, ariko bikagaragaza uburyo bushoboka bwo kwanduza hakiri kare kanseri zitandukanye zikoresha iyi mnyurari nyinshi kandi zigororotse.

ADN iva mu maraso yaturutse ku barwayi 31 ba leukemia n'abantu 12 bazima barasesenguwe. Nkuko bigaragara mu gasanduku umugambi mu gishushanyo cya 2a, gushishikarira kwa kanseri y'icyitegererezo cya kanseri (ΔA650 / 525) byari munsi ya ADN kuva ku ngero zisanzwe. Ibi byatewe ahanini na hydrophobicity itezimbere kwiza kwa kanseri ADN, yabujije igiteranyo cya Cyst / Aduns. Kubera iyo mpamvu, aba Nanoparticles bakwirakwijwe rwose mubice byo hanze bya kanseri yaka umuriro, byavuyemo gutanyagura ukundi, Aduns yaguye Asst Adna igizwe na ADN. Roc Imirongo noneho yakozwe muguhindura urwego rufite agaciro ka ΔA650 / 525 ku gaciro ntarengwa.

Igishushanyo 2. (a) Indangamuntu Yihariye Zibicuruzwa bya Cyst / Gufungura ibisubizo byerekana ko hariho ibisanzwe (ubururu) na kanseri (umutuku) ADN

(Da650 / 525) y'ibibanza; (b) Isesengura rya ROC no gusuzuma ibizamini byo gusuzuma. . .

Nkuko bigaragara ku gishushanyo cya 2b, agace kari munsi ya roc (auc = 0.9274) yabonetse kuri sensor yateje imbere yerekanye ubushishozi bwinshi kandi bwihariye. Nkuko bigaragara mu gasanduku umugambi, ingingo yo hasi igereranya itsinda risanzwe rya ADN ntabwo ryatandukanijwe neza nitsinda ryinshi ryerekana itsinda rya kanseri; Kubwibyo, gusubira inyuma byakoreshejwe mugutandukanya amatsinda asanzwe na kanseri. Urebye urutonde rwibihinduka byigenga, bigereranya amahirwe yibyabaye bibaye, nka kanseri cyangwa itsinda risanzwe. Ingaruka zishingiye kuri 0 na 1. Igisubizo rero ni amahirwe. Twagennye amahirwe yo kumenyekanisha kanseri (p) ashingiye kuri ΔA650 / 525 ku buryo bukurikira.

aho b = 5.3533, w1 = -6.965. Kubyitegererezo byicyitegererezo, amahirwe yo kuba munsi ya 0.5 yerekana icyitegererezo gisanzwe, mugihe bishoboka 0.5 cyangwa hejuru cyerekana icyitegererezo cya kanseri. Igishushanyo 2C cyerekana urujijo Matrix yavuye mu kiruhuko - cyonyine cyambukiranya, byakoreshwaga mu kwemeza umutekano wuburyo bwo gukwirakwiza. Igishushanyo cya 2d incamake isuzuma ryikizamini cyo gusuzuma uburyo, harimo sensitivite, umwihariko, agaciro keza (PPV) hamwe nagaciro kateganijwe (NPV).

Amaterefone-alies

Kugira ngo ukomeze kwipimisha icyitegererezo nta gukoresha ibintu bifatika, abashakashatsi bakoresheje ubwenge bw'ubukorikori (AI) kugira ngo basobanure ibara ry'igisubizo no gutandukanya abantu basanzwe ndetse na ba kanseri. Urebye ibi, iyerekwa rya mudasobwa ryakoreshejwe muguhindura ibara ryibiti bya Cyst / Gutanga igisubizo gisanzwe (ibara ry'umuyugubwe) cyangwa umutuku (umutuku) ukoresheje amashusho y'isahani ya terefone igendanwa. Ubwenge bwubuhanga burashobora kugabanya ibiciro no kunoza uburyo bwo kugerwaho mugusobanura ibara ryibisubizo bya Nanoparticle, kandi nta ikoreshwa ryibikoresho byose bya optal. Hanyuma, moderi ebyiri zikoresha imashini, harimo n'ishyamba ritoroshye (RF) no gushyigikira imashini ya Vector (SVM) zatojwe kubaka icyitegererezo. Byombi rf na svm byitondeye neza ingero nziza kandi mbi hamwe nukuri 90.0%. Ibi byerekana ko gukoresha ubwenge bwubukorikori muri bioking deltueling ya terefone igendanwa birashoboka.

Igicapo 3. (a) Icyiciro cyicyiciro cyigisubizo cyanditswe mugihe cyo gutegura icyitegererezo cyo guterana ibitekerezo. (b) Urugero rwafashwe mugihe cyo gushaka ishusho. .

Gukoresha Cyst / Aduns, abashakashatsi batsimbataje neza urubuga rworoshye rwo gutahura umuyoboro wa methilelation hamwe na sensor ishoboye gutandukanya dna isanzwe iva muri kanseri mugihe bakoresheje amaraso nyayo kugirango basuzume amaraso. Umusekezi wateye imbere yerekanye ko ADN yakuyeho ingero zukuri zamaraso nyayo yashoboye vuba kandi igura-kanseri ntoya ya kanseri (3nm) mumashuri ya leukemia muminota 15, kandi yerekanaga ukuri 95.3%. Kugirango ukomeze kwipimisha icyitegererezo ukuraho gukenera imashini zikenewe mu gusobanura ibara no gutandukanya abantu basanzwe kandi batandukanye bakoresheje ifoto ya terefone igendanwa, kandi narashobora kugerwaho kuri 90.0%.

Reba: Doi: 10.1039 / D2RA05725E