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Mu mirima yagusuzuma indwara mu buryo bwa "clinical in vitro diagnostics" (IVD), gusuzuma imiterere y'uturemangingo tw'umubiri, n'ubushakashatsi ku ngirabuzimafatizo, ingero zo mu kanwa—nk'ukoudupira two mu kanwa, udupira two mu muhogo, n'amacandwe—bikoreshwa cyane mu gupima aside nucleique bitewe nukogukusanya byoroshye, imiterere idatera indwara, kandi inzira yo gufatira ingero nta bubabareAriko, ingero zo mu kanwa akenshi ziba zirimoingano nke ya aside nucleiquekandi akenshi banduzwa naporoteyine n'indi myandaUburyo gakondo bwo gukuramo ibintu bukunze kugira ingaruka mbi kuimikorere igoye, imikorere mike, no gukoresha ibikoresho by'uburozi, bishobora kwangiza cyane uburyo bwo gukora neza no kudahungabana kw'ikoreshwa rya porogaramu zo muPCR/qPCR n'uburyo bwo gukurikirana ibipimo by'ibizamini by'abakiri bato (NGS).
ItsindaBFMP06 Magnetic Bead Oral Swab Genomic DNA Extraction Kit, byakozwe naIkoranabuhanga rya Biotechnology rya Hangzhou Bigfish FeiXu, itangaigisubizo cyizewe, cyiza kandi cyizewegukurura ADN mu kanwa. Kubera imiterere yayo mishya ya tekiniki n'ibipimo by'imikorere bihamye, iyi mashini yabaye igikoresho cyizewe haba muri laboratwari z'ubuvuzi n'iz'ubushakashatsi.
Igikoresho cya BFMP06 cyubatswe hakurikijwesisitemu ya buffer igezweho cyanehamwe naAmasaro ya hydroxyl magnetic yihariye ya ADN, bigakora imikorere myiza cyane yo gutunganya aside nucleique. Nyuma y'uko icyitegererezo gishyizwe muri lysis buffer, ibice by'uturemangingo birahungabana maze aside nucleique zikarekurwa. Amatsinda y'imikorere ari ku buso bw'udupira twa rukuruzi ahuza neza ADN, bigatuma ihinduka ihamye.urusaku rwa magnetic bead–DNA complexes.
Munsi y'imbaraga za rukuruzi zo hanze, izo complexes zinyuramointambwe ebyiri zo gukaraba nezagukuraho neza poroteyine, umunyu, n'ibindi bintu bihumanya. Amaherezo,ADN y'uturemangingo tw'umubiri ifite ubwiza bwinshiirakurwaho neza hakoreshejwe alubute ya elution.
Ibisobanuro by'igicuruzwa
Iki gicuruzwa gikoresha sisitemu yihariye kandi igezweho yo gufunga hamwe naamasaro ya rukuruzi ahuza by'umwihariko ADN, bigatuma aside nucleike ihuzwa vuba, igatandukana, kandi igasukurwa. Irakwiriye cyane kuGutandukanya ADN y’uturemangingo tw’amaraso mu buryo bwihuse kandi bunoze n’udupfundikizo two mu kanwa, udupfundikizo two mu muhogo, n’ibipimo by’amacandwe., mu gihe bikuraho neza poroteyine n'imyunyu bisigaye.
Iyo ikoreshejwe hamwe naIbikoresho byo gukuramo aside nucleique ishingiye kuri Bigfish FeiXu, iyi kit ni nziza cyane kurigusohora ibikoresho byikora ku buryo bworoshyeADN ya genome isukuye ni iyaubuziranenge bwinshi n'ubwiza buhebuje, bigatuma ikoreshwa mu buryo butandukanye, harimoPCR/qPCR na NGS.
Ibiranga Ibicuruzwa
Ubwiza bwo hejuru
Itandukanya neza kandi igasukura ADN ya genome muriudupira two mu kanwa, udupira two mu muhogo, n'amacandwegutangaumusaruro mwinshi n'ubuziranenge bwinshi.
Byihuse kandi byoroshye
Nta ntambwe zo gupima amashanyarazi cyangwa kuyungurura umwuka zisabwa. Bijyanye n'ibikoresho byo gukuramo ibikoresho byikora, bigatuma biba byiza kurigutunganya ingero nini.
Ifite umutekano kandi ntigira uburozi
Nta mpamvu yo gukoresha ibintu bihumanya nk'ibikomoka ku bimera nkafenoli cyangwa chloroform.
Ibikoresho Bihuye
Bigfish FeiXu BFEX-16E
BFEX-32
BFEX-32E
BFEX-96
Ibisubizo by'igerageza
Ingero z'umuti wo mu kanwa (winjijwe mu400 μL umuti wo kubungabunga) n'ibipimo by'amacandwe (Umuti wo kubika amacandwe wa 200 μL + 200 μL) byatunganyijwe hakoreshejweBigfish FeiXu Oral Swab Genomic DNA Purification KitADN yabuze muri70 μL alution bufferkandi yasesenguwe naelectrophoresis ya agarose gel, nk'uko bigaragara ku ishusho iri hepfo.
M: Ikimenyetso cya ADN (2K Plus II)
Ibisobanuro by'ibicuruzwa
Igihe cyo kohereza: Mutarama 15-2026
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