Amakuru - Ikimenyetso Gishya cyo Gukura ADN y'Amazi mu Bidukikije — Bigefei Sequencing Yihutisha Ubushakashatsi bwa Siyansi

Uburyo bwa Magnetic Bead bukemura neza ibibazo byo gukura ADN y'amazi mu bidukikije

Mu nzego nko mu bushakashatsi ku bidukikije no kugenzura ihumana ry’amazi, gukuramo ADN nziza ya genomic ni ngombwa cyane kugira ngo habeho ikoreshwa rya PCR/qPCR na next-generation sequencing (NGS). Ariko, ingero z’amazi akoreshwa mu bidukikije ziragoye cyane, zirimo udukoko dutandukanye, ubwoko bugoye gukoresha nka bagiteri za Gram-positive, ndetse n’imbogamizi zimaze igihe kirekire zijyanye n’uburyo gakondo bwo gukuramo—nk’ikoreshwa ry’ibintu bihumanya n’uburyo bugoye—byakomeje guteza ibibazo abashakashatsi.

Ubu, Bigfish Sequencing izanye BFMP24R Magnetic Bead-Based Environmental Water Genomic DNA Extraction and Purification Kit, itanga igisubizo cyuzuye kuri ibi bibazo binyuze mu ikoranabuhanga rigezweho no gushushanya byoroshye gukoresha.

Incamake y'ibicuruzwa

Iyi seti ishingiye kuri sisitemu nziza yo gufunga ivanze n'udupira twa nano magnetic dufite imikorere myiza. ADN ya genomike ihuza by'umwihariko n'amatsinda imikorere ari ku buso bw'udupira kandi igatandukanywa n'imbaraga za rukuruzi zo hanze. Nyuma yo gukaraba buhoro buhoro kugira ngo poroteyine, imyunyu, n'indi myanda, ADN ya genomike ifite isuku nyinshi iracika burundu.

Iyi mashini yagenewe by'umwihariko ikoreshwa mu gupima amazi aturuka ku bidukikije, ikuramo neza ADN ya bagiteri yakusanyijwe ku ruhu rw'amazi, harimo bagiteri za Gram-negative na Gram-positive (kugeza ku tunyangingo twa bagiteri 2 × 10⁹ kuri ruhu rw'amazi rumwe). Irahuye na sisitemu zo gukuramo aside nucleic zikora mu buryo bwikora kugira ngo itunganywe neza. ADN yakuwemo ifite ubuziranenge buhamye kandi ishobora gukoreshwa mu buryo butaziguye kuri PCR/qPCR, NGS, n'izindi porogaramu zo hasi.

Ibiranga Ibicuruzwa

1. Ubushobozi bwo gukuramo bagiteri mu buryo bwa Broad-Spectrum

Ikura neza bagiteri za Gram-negative na Gram-positive mu mazi, igakwirakwira mu duce duto dukunze kuboneka mu mazi meza no mu nyanja, kandi ikuzuza ibisabwa bitandukanye byo gusesengura.

2. Ubuziranenge bwinshi n'umusaruro mwinshi

Itanga ADN ifite ubuziranenge bwinshi, nta bintu biyibuza kwanduza, kandi itanga umusaruro uhoraho ukwiriye gukoreshwa mu buryo butaziguye mu miterere y’uturemangingo.

3. Guhuza ikoranabuhanga mu buryo bwikora kandi bunoze cyane

Ikorana neza na sisitemu zo gukuramo aside nucleic za Bigfish zikora, ifasha gutunganya icyarimwe ingero 32 cyangwa 96, bigabanya cyane igihe cyo kuzitunganya no kunoza imikorere ya laboratwari.

4. Imikorere myiza kandi yoroshye kuyikoresha

Nta mpamvu yo gukoresha ibintu bihumanya nk'ibikomoka kuri shimi nka phenol cyangwa chloroform, bigabanya ingaruka mbi ku mutekano wa laboratwari. Ibintu binini bishyirwa mu mapine y'amabati 96, bigabanya amakosa yo gushyira mu miyoboro y'amazi n'intoki kandi bikoroshya imikorere.

Ibikoresho Bihuye

Bigfish BFEX-16E

BFEX-32

BFEX-32E

BFEX-96E

Ibisubizo by'igerageza

Ingero y'amazi yo mu ruzi ya mililitiro 600 yayunguruwe binyuze mu ruhu, maze ADN ikurwamo hakoreshejwe uburyo bwa Bigfish magnetic bead based environmental water genomic DNA extraction and purification kit hamwe n'igikoresho gihuye nacyo. ADN yakuwemo yaje gusesengurwa hakoreshejwe agarose gel electrophoresis.

M: Ikimenyetso1, 2: Ingero z'amazi y'uruzi

Ibisobanuro by'ibicuruzwa

Igihe cyo kohereza: Ukuboza 18-2025