MagPure Bacteria Genomic ADN yo Gusukura

Ibisobanuro bigufi:

Nyuma yo kuvurwa n'amazi yo mu gifu, aside nucleic iri mu gipimo cya bagiteri irekurwa gusa hakoreshejwe Lysis Buffer. ADN ya genomic irekurwa ihambirwa gusa na Magnetic beads. ADN ya genomic ihambirwa ku duce twa magnetic ifatwa n'ibintu bya magnetic; imyanda ikurwaho no kozwa na Wash Buffer. Aside nucleic ikurwa mu duce hakoreshejwe Elution Buffer. Ikoreshwa ku bwoko bwose bwa bagiteri za gram-negative na bagiteri nziza, nibindi.

TWANDIKIRE

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Igice cy'ingenzi

Injangwe.#	BFMP10R1	BFMP10R16	BFMP10R	Cibintu
Pagukubita	1T40T/ibikoresho	16T16T/agasanduku	32T32T/ibikoresho
Protease K	400μ L x 2	320μ L	640μ L	Umuti wa poroteyine (Upakiye ukwawo)
RNaseA	80μ L	32μ L	64μ L	Umuti wa enzyme (Upakiye ukwawo)
Ishuri rikuru rya Buffer	12ml	5ml	10ml	Umuti w'umunyu
Buffer BL	Mu mirongo ine na gatandatu gutegura. karitoyi Ibice 40.	96 ikiraro byapakiwe mbere isahani Ibice 2.	96 ikiraro byapakiwe mbere isahani Ibice 2.	Denaturant ikomeye na Tris buffer
Buffer WA				Umuti w'umunyu
Ihuriro ry'amakuru (Buffer WB)				Umuti w'umunyu muke
Buffer DE				Igisubizo cya Tris
Amasaro y'ingufu				Umuti w'ubusaro bwa hydroxy magnetic
Igitabo cy'Abakoresha	1	1	1