Marcome amaraso ya dna ya dna

Ibisobanuro bigufi:

Ibi bikoresho birimo Microspitike Custoceres na FreePactions Buffer, kandi bikwiranye no gukora neza kandi neza gukuramo ADN ya gisanzwe, bikonje, kandi birebire byateganijwe bitemewe n'amaraso yose. Ibice bya ADN byakuweho bya DNN ni binini, byera cyane, kandi bifite ireme kandi byizewe. ADN yakuweho ikwiranye nubushakashatsi butandukanye bwamanutse nka Enzyme Dispetion, PCR, kubaka isomero, kuvanga amajyepfo, hamwe no gukurikiranya hirya no hino.

Twandikire

Ibisobanuro birambuye

Ibicuruzwa

Ibiranga ibicuruzwa

Urwego runini rwicyitegererezo:ADN ya Nenomic irashobora gukurwa mu buryo butaziguye mu buryo bw'amaraso adutiko (EDTA, HEPARIN, HET.), ikote ry'amaraso, n'amaraso.
Byihuta kandi byoroshye:Icyitegererezo lysis na aside iricleic bindide bikorwa icyarimwe. Nyuma yo gupakira icyitegererezo kuri mashini, gukuramo acide nucleic birarangiye mu buryo bwikora, kandi ADN yo hejuru ya none irashobora kuboneka muminota irenga 20.
Umutekano kandi utari uburozi:Reagent ntabwo ikubiyemo ibishushanyo byuburozi nka fenol na chloroforform, kandi ifite ikintu cyinshi cyumutekano.

Ibikoresho bifitanye isano

Bigfish Bfex-32e / Bfex-32 / Bfex-96E

Tekinike

Umubare w'icyitegererezo:200μl
ADNED:≧ 4μg
ADNE ITERY:A260 / 280 ≧ 1.75

Ibisobanuro

Izina ry'ibicuruzwa	Injangwe. Oya	Gupakira
Marcome amaraso ya anna ya dna yera (paki yuzuye)	Bfmp02r	32T
Marcome amaraso ya anna ya dna yera (paki yuzuye)	Bfmp02r1	40t
Marcome amaraso ya anna ya dna yera (paki yuzuye)	Bfmp02r96	96T