FFPE ibikoresho byo kweza ADN

FFPE ADN yo Gusukura Igikoresho cyerekanwe

Ibisobanuro bigufi:

Igikoresho gikoresha igisubizo cyangiza ibidukikije cyangiza ibidukikije kugirango gikuremo ADN nziza yo mu bwoko bwa ADN ikozwe mu ngero zashyizwemo paraffine, ikanesha ingaruka zo kubuzwa guterwa no guhuza imiyoboro ya foromine.
Icyitegererezo kivanze na selile lysis buffer kugirango isenye selile kandi igaragaze ADN. Icyitegererezo kivanze na selile lysis buffer kugirango tumenye selile kandi ugaragaze ADN. ADN yashyizwe ahagaragara ifatwa na magnetiki isaro ya nanoparticles kugirango ibe isaro-ADN. Amasaro-ADN yubatswe yimuriwe mumasaro-ADN yimurwa kugirango yoze buffer kugirango ikureho umwanda urenze. Hanyuma, ADN irasubizwa kandi igarurwa mukuyimura kuri buffer.

TWANDIKIRE

Ibicuruzwa birambuye

Ibicuruzwa

Ingero

Ibice bya FFPE, guhagarika FFPE, icyitegererezo mubisubizo bikosorwa nka formalin

Ibigize

Icyitegererezo	BFMP12R1	BFMP12R16	BFMP12R	Ibigize
Gupakira	1T40T / kit	16T16T / kit	32T32T / kit
Kurinda K.	400μL x 2	320μL	640μL	Igisubizo (Bipakiwe bitandukanye)
RNase A.	80μL	32μL	64μL	Enzyme igisubizo (Bipakiwe bitandukanye)
Buffer FA	12mL	5ml	10ml	Umuti mwinshi
Buffer FL	Muri mirongo ine 6-neza kwitegura. karitsiye 40 pc.	96 neza byapakiwe mbere isahani 2 pc	96 neza byapakiwe mbere isahani 2 pc.	Gukomera kwa denaturant na Tris buffer
Buffer WA				Umuti mwinshi
Buffer WB				Umuti muke wa saline
Buffer DE				Umuti muke wa saline
Amasaro ya rukuruzi				Hydroxy magnetic isaro yumuti
Igitabo cyabakoresha	1	1	1