2 × sybr Green QPCR ivanga (hamwe na rox ndende)

Ibicuruzwa biranga ibicuruzwa

Iki gicuruzwa, 2 × sybr icyatsi kibisi kirimo ibice byose bikubiyemo ibigize pcr, harimo icyatsi kibisi, dntps, mg2 +, na pcr buffer.
Sybr Icyatsi I Irangi irangi ryicyatsi kibisi buhuza ADN inshuro ebyiri (DSDNA) kabiri Ibi bituma bishoboka kugereranya ingano ya ADN inshuro ebyiri zakozwe mugihe cyongereranyo mugutahura ubukana bwa fluorescence.
Rox ikoreshwa nkirangi yo gukosora kugirango ikosore ihindagurika rya fluorescence ridafitanye isano na PCR, bityo rigabanya itandukaniro ryibice. Itandukaniro nkiryo rirashobora guterwa nibintu bitandukanye, nkikosa rya pipette cyangwa icyitegererezo. Ibikoresho bitandukanye bya fluorescence bifite ibyo akeneye kuri rox, kandi iki gicuruzwa gikwiranye no gusesengura ibinuye bisaba rox yo hejuru.