2 × SYBR green qPCR MIX （Ifite ROX nyinshi）

Ibiranga ibicuruzwa

Iki gicuruzwa, 2×SYBR green qPCR MIX, kiza mu muyoboro umwe urimo ibice byose bikenewe kugira ngo PCR yongere kandi imenyekane, harimo Taq DNA poLymerase, SYBR green I color, High ROX Reference Dye, dNTPs, Mg2+, na PCR buffer.
Irangi rya SYBR green I ni irangi ry'icyatsi kibisi rihuzwa na ADN ifite imigozi ibiri (DNA ifite imigozi ibiri, dsDNA) double helix minor groove region. SYBR green I fluoresces iragaragara mu buryo budakomeye mu gihe cy'ubusa, ariko iyo imaze gufatana na ADN ifite imigozi ibiri, fluorescence yayo irushaho kwiyongera cyane. Ibi bituma bishoboka gupima ingano ya ADN ifite imigozi ibiri ikorwa mu gihe cyo kongera imbaraga za PCR binyuze mu kumenya ubukana bwa fluorescence.
ROX ikoreshwa nk'irangi ryo gukosora kugira ngo ikosore ihindagurika ry'urumuri ridafite aho rihuriye na PCR, bityo bigabanye itandukaniro ry'ahantu. Itandukaniro nk'iryo rishobora guterwa n'ibintu bitandukanye, nko gukosa kuri pipette cyangwa gushonga kw'icyitegererezo. Ibikoresho bitandukanye byo gupima urumuri bifite ibyo ROX ikenera bitandukanye, kandi iki gicuruzwa kibereye abasesengura ingano y'urumuri bakeneye gukosora ROX cyane.