2 × SYBR icyatsi kibisi qPCR MIX （Hamwe na ROX yo hejuru）

Ibiranga ibicuruzwa

Iki gicuruzwa, 2 × SYBR icyatsi kibisi qPCR MIX, kiza mu muyoboro umwe urimo ibice byose bisabwa kugira ngo PCR yongerwe imbaraga kandi imenyekane, harimo Taq ADN poLymerase, SYBR icyatsi kibisi I irangi, Irangi ROX ryerekana irangi, dNTPs, Mg2 +, na PCR buffer.
SYBR icyatsi I Irangi ni irangi ryicyatsi kibisi gihuza ADN ebyiri (ADN ya kabiri, dsDNA) agace ka helix ntoya ya shobuja. , fluorescence yayo yazamutse cyane. Ibi bituma bishoboka kugereranya ingano ya ADN ikubye kabiri yakozwe mugihe cya PCR yongerewe imbaraga mukumenya ubukana bwa fluorescence.
ROX ikoreshwa nk'irangi ryo gukosora kugirango ikosore ihindagurika rya fluorescence ridafitanye isano na PCR, bityo bigabanye itandukaniro ryaho. Itandukaniro nkiryo rishobora guterwa nimpamvu zitandukanye, nkikosa rya pipette cyangwa sample evaporation. Ibikoresho bitandukanye byo kugereranya fluorescence bifite ibikenerwa bitandukanye kuri ROX, kandi iki gicuruzwa gikwiranye nisesengura rya fluorescence risaba gukosorwa ROX.